Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody

Home > Product > Antibody > Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody

Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF7A; IRF7; IRF7H.

Cat:

SL3196R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472:GV(p-S)(p-S)LD

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500Flow-Cyt=1ug/testICC=1:100（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Uterus (Mouse) Lysate at 40 ugLarge intestine (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54kDObserved band size: 49kDSample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Thymus tissue lysates Lane 3: Mouse Blood cell lysates Lane 4: Mouse Lung tissue lysates Lane 5: Rat Spleen tissue lysates Lane 6: Rat Liver tissue lysates Lane 7: Human Jurkat cell lysates Lane 8: Human Raji cell lysates Primary: Anti-Phospho-IRF7 (Ser471 + Ser472) (SL3196R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 70,55 kDSample:Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54kDObserved band size: 48kDSample:Spleen (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54kDObserved band size: 49kDSample: Heart(Mouse) lysate at 30ug; Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (SL3196R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 54kDObserved band size : 51kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (SL3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (SL3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (SL3196R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (SL3196R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008]

Function:
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.

Subunit:
Homodimer.

Subcellular Location:
Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.

Tissue Specificity:
Expressed predominantly in spleen, thymus and peripheral blood leukocytes.

Post-translational modifications:
In response to a viral infection, phosphorylated on the SLCterminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
TRAF6-mediated ubiquitination is required for IRF7 activation.

Similarity:
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

SWISS:
Q92985

Gene ID:
3665

Database links:

Entrez Gene: 3665 Human

Entrez Gene: 54123 Mouse

Entrez Gene: 293624 Rat

Omim: 605047 Human

SwissProt: Q92985 Human

SwissProt: P70434 Mouse

Unigene: 166120 Human

Unigene: 3233 Mouse

Unigene: 101159 Rat

Picture

Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Thymus tissue lysates
Lane 3: Mouse Blood cell lysates
Lane 4: Mouse Lung tissue lysates
Lane 5: Rat Spleen tissue lysates
Lane 6: Rat Liver tissue lysates
Lane 7: Human Jurkat cell lysates
Lane 8: Human Raji cell lysates
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472) (SL3196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 70,55 kD

Sample:Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 48kD

Sample:Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 49kD

Sample: Heart(Mouse) lysate at 30ug;
Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (SL3196R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size : 54kD
Observed band size : 51kD

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (SL3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (SL3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (SL3196R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (SL3196R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall