May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Function:
The precursor protein but not the mature protein may form multimers.
Subunit:
The precursor protein but not the mature protein may form multimers.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Post-translational modifications:
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Similarity:
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
SWISS:
Q8NBP7
Gene ID:
255738
Database links:
Entrez Gene: 255738 Human
Entrez Gene: 100102 Mouse
Entrez Gene: 298296 Rat
Omim: 607786 Human
SwissProt: Q8NBP7 Human
SwissProt: Q80W65 Mouse
SwissProt: P59996 Rat
Unigene: 18844 Human
Unigene: 133268 Mouse
Unigene: 19195 Rat
前蛋白转化酶枯草溶菌素9(PCSK9)是近年来新发现的一种与调节脂质代谢密切相关的基因,编码前蛋白转化酶NARSLC1。Narc-1/pcsk9可通过调节细胞表面的低密度脂蛋白受体(LDLR)水平,参与脂质代谢,从而在动脉粥样硬化性疾病的发生、发展中发挥重要作用。
| Picture |
Sample:
Lane 1:A431(Human) Cell Lysate at 30 ug
Lane 2:Uterus (Mouse) Lysate at 40 ug
Primary: Anti- NARC1/PCSK9 (SL6060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 70 kD
Sample:
Lane 1: HepG2(Human) Cell Lysate at 30 ug
Lane 2: SW96(Human) Cell Lysate at 30 ug
Primary: Anti- NARC1/PCSK9 (SL6060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (SL6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (SL6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (NARC1/PCSK9) polyclonal Antibody, Unconjugated (SL6060R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-NARC1/PCSK9 antibody (SL6060R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
|
|
