Rabbit Anti-CD81 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-CD81 antibody

26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tet

Cat:

SL6934R

Species Reactivity：

Human,Mouse,(predicted: Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TAPA1/CD81:101-210/236<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1μg/TestIF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Lymph node (Mouse) Lysate at 40 ugLane 2: Spleen (Mouse) Lysate at 40 ugLane 3: Lung (Mouse) Lysate at 40 ugLane 4: Thymus (Mouse) Lysate at 40 ugLane 5: Raw264.7 (Mouse) Cell Lysate at 30 ugLane 6: THP-1 (Human) Cell Lysate at 30 ugLane 7: Raji (Human) Cell Lysate at 30 ugPrimary: Anti-CD81 (SL6934R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 25 kDSample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-CD81 (SL6934R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 26 kDSample: U937(Human) Cell Lysate at 30 ugPrimary: Anti-CD81 (SL6934R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 26 kDSample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti-CD81 (SL6934R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 26 kDSample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-CD81 (SL6934R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 25 kDBlank control(blue): Jurkat cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD81 antibody(SL6934R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Blank control: Mouse Brain cells(blue). Primary Antibody: Rabbit Anti- CD81 /PEConjugated antibody (SL6934R-PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008].

Function:
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.

Subunit:
Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Interacts with CD53 and SCIMP.

Subcellular Location:
Membrane.

Tissue Specificity:
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

DISEASE:
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

Similarity:
Belongs to the tetraspanin (TM4SF) family.

SWISS:
P60033

Gene ID:
975

Database links:

Entrez Gene: 975 Human

Omim: 186845 Human

SwissProt: P60033 Human

Unigene: 54457 Human

Picture

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD81 (SL6934R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD

Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti-CD81 (SL6934R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-CD81 (SL6934R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD

Sample: Lung (Mouse) Lysate at 40 ug
Primary: Anti-CD81 (SL6934R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 25 kD

Blank control(blue): Jurkat cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD81 antibody(SL6934R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Blank control: Mouse Brain cells(blue).
Primary Antibody: Rabbit Anti- CD81 /PEConjugated antibody (SL6934R-PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.

Product Feedback Wall

Specific References (13) | SL6934R has been referenced in 13 publications.

[IF=3.786] Yinfeng Chen. et al. PLA2G10 incorporated in exosomes could be diagnostic and prognostic biomarker for non-small cell lung cancer. Clin Chim Acta. 2022 Feb;: WB ; Human.

PubMed:35231479

[IF=3.281] Yinfeng Chen. et al. Serum exosomal hsa_circ_0069313 has a potential to diagnose more aggressive non-small cell lung cancer. Clin Biochem. 2022 Jan;: WB ; Human.

PubMed:35077682

[IF=10.435] Li, Kanglu. et al. Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages. J Nanobiotechnol. 2022 Dec;20(1):1-20 WB ; Human.

PubMed:35057811

[IF=6.639] Hyesun Jeong. et al. GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer. Cancers. 2021 Jan;13(21):5482 WB ; Human.

PubMed:34771645

[IF=9.518] Fan Y et al. High-sensitive and multiplex biosensing assay of NSCLSLCderived exosomes via different recognition sites based on SPRi array. Biosens Bioelectron. 2020 Apr 15;154:112066. WB&SPRi biosensor ; Human.

PubMed:6456961

[IF=8.063] Faruqu FN et al. Membrane radiolabelling of exosomes for comparative biodistribution analysis in immunocompetent and immunodeficient mice–a novel and universal approach. Theranostics. 2019 Feb 28;9(6):1666-1682. FCM ; Mouse.

PubMed:31037130

[IF=5.923] Samanta Mecocci. et al. Transcriptomic Characterization of Cow, Donkey and Goat Milk Extracellular Vesicles Reveals Their Anti-Inflammatory and Immunomodulatory Potential. Int J Mol Sci. 2021 Jan;22(23):12759 WB ; Cow, Donkey,Goat.

PubMed:34884564

[IF=4.599] Shen, Xintong. et al. Evaluation of EpCAM-specific exosomal lncRNAs as potential diagnostic biomarkers for lung cancer using droplet digital PCR. 2021 Oct 14 WB ; Human.

PubMed:34651202

[IF=7.711] Xiaoyi Gao. et al. Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. Acs Sensors. 2021;6(10):3611–3620 FC ; Human.

PubMed:34632781

[IF=5.696] Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692 WB ; Human.

PubMed:34153644

[IF=4.546] Samanta Mecocci. et al. Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile. Nutrients. 2020 Oct;12(10):2908 WB ; Cow, Goat, Donkey.

PubMed:32977543

[IF=3.998] Jik-Han Jung. et al. Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma. Sci Rep-Uk. 2021; 11: 217 WB ; Human.

PubMed:33436891

[IF=0] Blans et al. Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography. (2017) J.Extracell.Vesicle. 6:1294340 WB ; Human.

PubMed:28386391