Rabbit Anti-BDNF antibody | Sunlong Medical

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Abrineurin; BDNF; BDNF; BDNF_HUMAN; Brain Derived Neurotrophic Factor; Brain-derived neurotrophic factor; MGC34632; MGC34632; Neurotrophin.

Cat:

SL4989R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human BDNF:151-247/247

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:200-800（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugCerebellum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-BDNF (SL4989R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 13/27 kDObserved band size: 18/32 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.Tissue/cell: BSLV2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.Blank control(blue): HepG2(fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody:Rabbit Anti-BDNF antibody (SL4989R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. More recently, three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4), also designated NT-5. These various neurotrophins stimulate the in vitro survival of distinct but partially overlapping populations of neurons. The Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds equally well to both BDNF and NT-4 and to a lesser extent NT-3, while the Trk C receptor only binds NT-3. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Belongs to the NGF-beta family.

Function:
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Subunit:
Monomers and homodimers. Binds to NTRK2/TRKB.

Subcellular Location:
Secreted.

Tissue Specificity:
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Post-translational modifications:
The propeptide is N-glycosylated and glycosulfated.

DISEASE:
Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209176]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.

Similarity:
Belongs to the NGF-beta family.

SWISS:
P23560

Gene ID:
627

Database links:

Entrez Gene: 627 Human

Entrez Gene: 12064 Mouse

Entrez Gene: 24225 Rat

Omim: 113505 Human

SwissProt: P23560 Human

SwissProt: P21237 Mouse

SwissProt: P23363 Rat

Unigene: 502182 Human

Unigene: 1442 Mouse

Unigene: 11266 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.

Tissue/cell: BSLV2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (SL4989R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Blank control(blue): HepG2(fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-BDNF antibody (SL4989R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Specific References (9) | SL4989R has been referenced in 9 publications.

[IF=5.33] YuanYuan Hou. et al. Long-term variable photoperiod exposure impairs the mPFC and induces anxiety and depression-like behavior in male wistar rats. Exp Neurol. 2022 Jan;347:113908 IHC ; Rat.

PubMed:34710402

[IF=4.225] Liu P et al. Harmine Ameliorates Cognitive Impairment by Inhibiting NLRP3 Inflammasome Activation and Enhancing the BDNF/TrkB Signaling Pathway in STZ-Induced Diabetic Rats. Front Pharmacol. 2020 May 1;11:535. WB/IF ; Rat.

PubMed:32425784

[IF=2.367] Sheen JM et al. Young rats with increased circulatory asymmetric dimethylarginine exhibited spatial deficit and alterations in dorsal hippocampus brain-derived neurotrophic factor and asymmetric dimethylarginine: Effects of melatonin. Int J Dev Neurosci. 2019 Sep 21;78:83-89. IHF ; Rat.

PubMed:31550497

[IF=4.77] Xu J. Y. et al. The Antidepressant-Like Effect of Vanillin Aroma Involves Serum Magnesium and Brain BDNF. (2018)Neuropsychiatry (London) Volume 8, Issue 4 IHC ; Rat.

PubMed:doi:10.4172/neuropsychiatry.1000470

[IF=3.26] Nader, Manar A., et al. "Sitagliptin enhances the neuroprotective effect of pregabalin against pentylenetetrazole-induced acute epileptogenesis in mice: Implication of oxidative, inflammatory, apoptotic and autophagy pathways." Neurochemistry International(2017). IHSLCP ; Mouse.

PubMed:2906411

[IF=5.62] Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014). WB ; Rat.

PubMed:24780497

[IF=1.782] Shrestha S et al. Differential regulation of brain‐derived neurotrophic factor (BDNF) expression in sensory neuron axons by mi RNA‐206. FEBS Open Bio,2018 9(2), 374–383. WB ; Rat.

PubMed:10.1002/2211-5463.12581

[IF=2.94] Zabłocka et al. Neurotrophic Activity of Cultured Cell Line U87 is Up-Regulated by Proline-Rich Polypeptide Complex and Its Constituent Nonapeptide. (2015) Cell.Mol.Neurobiol. 35:977-86 WB ;

PubMed:25841889

[IF=1.77] Xing et al. Non-viral liposome-mediated transfer of brain-derived neurotrophic factor across the blood-brain barrier. (2016) Neural.Regen.Re. 11:617-22 IF(IHSLCP) ; Rat.

PubMed:27212923