Rabbit Anti-Occludin antibody

Home > Product > Antibody > Rabbit Anti-Occludin antibody

Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin.

Cat:

SL10011R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Occludin:151-250/522<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2 Cell (Human) Lysate at 40 ugPrimary: Anti-Occludin (SL10011R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 59 kDObserved band size: 60 kDSample: Lane 1: A549 (Human) Cell Lysate at 30 ugLane 2: HepG2 (Human) Cell Lysate at 30 ugLane 3: Hela (Human) Cell Lysate at 30 ugPrimary: Anti-Occludin (SL10011R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65/55/38/28 kDObserved band size: 62/52/40/25 kDSample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-Occludin (SL10011R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 59 kDObserved band size: 59 kDSample: Testis (Mouse) Lysate at 40 ugKidney (Mouse) Lysate at 40 ugPrimary: Anti-Occludin (SL10011R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 59 kDObserved band size: 59 kDParaformaldehyde-fixed, paraffin embedded (Normal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated (SL10011R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Normal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated (SL10011R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (SL10011R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Occludin antibody (SL10011R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-Occludin antibody (SL10011R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].

Function:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subunit:
Interacts with TJP1/ZO1 and with VAPA.

Subcellular Location:
Membrane; Multi-pass membrane protein. Cell junction, tight junction.

Tissue Specificity:
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts.

Similarity:
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain.

SWISS:
Q16625

Gene ID:
100506658

Database links:

Entrez Gene: 18260 Mouse

Entrez Gene: 100506658 Human

Entrez Gene: 397236 Pig

Entrez Gene: 83497 Rat

Entrez Gene: 403844 Dog

Omim: 602876 Human

SwissProt: Q28269 Dog

SwissProt: Q16625 Human

SwissProt: Q61146 Mouse

SwissProt: Q6P6T5 Rat

Unigene: 592605 Human

Unigene: 967 Mouse

Unigene: 31429 Rat

Picture

Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: HepG2 (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-Occludin (SL10011R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65/55/38/28 kD
Observed band size: 62/52/40/25 kD

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Occludin (SL10011R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD

Sample:
Testis (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Occludin (SL10011R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD

Paraformaldehyde-fixed, paraffin embedded (Normal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated (SL10011R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (SL10011R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-Occludin antibody (SL10011R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-Occludin antibody (SL10011R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (34) | SL10011R has been referenced in 34 publications.

[IF=2.217] Qi Wang. et al. Effect of oral administration of Limosilactobacillus reuteri on intestinal barrier function and mucosal immunity of suckling piglets. Ital J Anim Sci. 2022;21(1):612-623 WB ; Piglets.

PubMed:10.1080/1828051X.2022.2048977

[IF=4.034] Fuqing Huang. et al. Nisin Z attenuates lipopolysaccharide-induced mastitis by inhibiting the ERK1/2 and p38 mitogen-activated protein kinase signaling pathways. J Dairy Sci. 2022 Feb;: WB ; Mouse.

PubMed:35181137

[IF=3.247] D.L. Wu. et al. Dietary supplementation of free or two fat-coated sodium butyrate with varying release times on gastrointestinal development and tight junctions in preweaning Holstein calves. Anim Feed Sci Tech. 2022 Mar;285:115224 WB ; Cow.

PubMed:10.1016/j.anifeedsci.2022.115224

[IF=2.043] Zhang Jin-Ming. et al. BRCC36 promotes intestinal mucosal barrier injury caused by BMP2 after ischemia-reperfusion via inhibiting PPARγ signaling. Biosci Biotech Bioch. 2021 Dec;: Mouse.

PubMed:34888627

[IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965 WB ; Duck.

PubMed:34775344

[IF=6.823] Caijun Zhao. et al. Aryl hydrocarbon receptor activation by Lactobacillus reuteri tryptophan metabolism alleviates Escherichia coli-induced mastitis in mice. Plos Pathog. 2021 Jul;17(7):e1009774 IHC ; Mouse.

PubMed:34297785

[IF=3.267] Juan Zhang. et al. The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis. Molecules. 2021 Jan;26(14):4149 IHC ; Mouse.

PubMed:34299424

[IF=4.546] Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against γ-Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816 IHC ; Mouse.

PubMed:33801268

[IF=5.546] Yaqi Chang. et al. Glucagon‐like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032 IHC ; Pig.

PubMed:32960454

[IF=3.212] Ting Zheng. et al. Combination of Enteral Nutrition and Probiotics Promote Recovery Following Ileal Pouch–Anal Anastomosis in Rats. 2020 Nov 05 IHC ; Rat.

PubMed:33150540

[IF=1.813] Zhang Boxun. et al. The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota. Evid-Based Compl Alt. 2020;2020:4568629 IHC ; Rat.

PubMed:33224253

[IF=4.067] Wang X et al. LRRC75A antisense lncRNA1 knockout attenuates inflammatory responses of bovine mammary epithelial cells. International Journal of Biological Sciences. 2020; 16(2): 251-263. WB&ICF ; Bovine.

PubMed:doi:10.7150/ijbs.38214

[IF=1.773] Qu XY et al. XingNaoJing injections protect against cerebral ischemia/reperfusion injury and alleviate blood-brain barrier disruption in rats, through an underlying mechanism of NLRP3 inflammasomes suppression. Chin J Nat Med. 2019 Jul;17(7):498-505. WB&IHSLCP ; Rat.

PubMed:31514981

[IF=2.118] Li X et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019 Apr 5;7:e6715. WB ; Bovine.

PubMed:30984483

[IF=1.607] Zhao B et al. N‐Acetyl‐d‐galactosamine prevents soya bean agglutinin‐induced intestinal barrier dysfunction in intestinal porcine epithelial cells. J Anim Physiol Anim Nutr (Berl). 2019 Apr 1. WB ; Porcine.

PubMed:30934149

[IF=3.289] Chen M et al. Dietary L-Tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in Caco-2 cell monolayer model.Food Funct. 2019 May 22;10(5):2390-2398. WB ; Human.

PubMed:30977499

[IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017). IHSLCP ; Mouse.

PubMed:29202267

[IF=4.546] Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863 WB ; Pig.

PubMed:34941701

[IF=7.963] Jun Yan. et al. Intestinal toxicity of micro- and nano-particles of foodborne titanium dioxide in juvenile mice: Disorders of gut microbiota–host co-metabolites and intestinal barrier damage. Sci Total Environ. 2022 May;821:153279 IHC ; Mouse.

PubMed:35074372

[IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188 WB ; Mouse.

PubMed:34875328

[IF=4.432] Juan Zhang. et al. Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora. Eur J Pharmacol. 2022 Feb;916:174730 IHC ; Mouse.

PubMed:34968462

[IF=10.479] Shiyun Chen. et al. Targeted delivery of Chinese herb pair-based berberine/tannin acid self-assemblies for the treatment of ulcerative colitis. J Adv Res. 2021 Nov;: IF ; Mouse.

PubMed:10.1016/j.jare.2021.11.017

[IF=9.229] Xiao Wang. et al. Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX IHC ; Mouse.

PubMed:34261306

[IF=4.013] Xia Liu. et al. Luteolin alleviates non-alcoholic fatty liver disease in rats via restoration of intestinal mucosal barrier damage and microbiota imbalance involving in gut-liver axis. Arch Biochem Biophys. 2021 Oct;711:109019 WB,IHC ; Rat.

PubMed:34478730

[IF=4.407] Caijun Zhao. et al. Kynurenic acid protects against mastitis in mice by ameliorating inflammatory responses and enhancing blood-milk barrier integrity. Mol Immunol. 2021 Sep;137:134 WB ; Mouse.

PubMed:34247099

[IF=3.531] Yanan Gao. et al. Aflatoxin B1 and Aflatoxin M1 Induce Compromised Intestinal Integrity through Clathrin-Mediated Endocytosis. Toxins. 2021 Mar;13(3):184 IF ; Mouse.

PubMed:33801329

[IF=3.375] Zheng Chen. et al. Glycine attenuates cerebrovascular remodeling via glycine receptor alpha 2 and vascular endothelial growth factor receptor 2 after stroke. Am J Transl Res. 2020; 12(10): 6895–6907 IF,IHC ; Rat.

PubMed:33194080

[IF=2.639] Luo Q et al. Iron Overload Resulting from the Chronic Oral Administration of Ferric Citrate Impairs Intestinal Immune and Barrier in Mice. Biol Trace Elem Res . 2020 May 28. WB ; Mouse.

PubMed:32468223

[IF=2.573] Kai-Yu Li et al. A New Rat Model of Pouchitis After Proctocolectomy and Ileal Pouch-Anal Anastomosis Using 2,4,6-Trinitrobenzene Sulfonic Acid. J Gastrointest Surg. 2020 May 18.

PubMed:32424688

[IF=3.392] Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3). WB ; Mouse.

PubMed:30897784

[IF=3.923] Yang Y et al. Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR. (2018) J Cell Physiol. IHF&WB ; Mouse.

PubMed:30515795

[IF=3.118] Ye J et al. The mTORC1 signaling modulated by intracellular C3 activation in Paneth cells promotes intestinal epithelial regeneration during acute injury.(2018) Int Immunopharmacol. IHF ; Mouse.

PubMed:30530169

[IF=3.166] Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. WB ; Piglet.

PubMed:3005548

[IF=2.959] Xu T et al. IL-1β induces increased tight junction permeability in bovine mammary epithelial cells via the IL-1β-ERK1/2-MLCK axis upon blood-milk barrier damage.J Cell Biochem. 2018 Nov;119(11):9028-9041. ICF&WB ; Cow.

PubMed:30011095