Rabbit Anti-GLUT2 antibody | Sunlong Medical

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liver; Glucose Transporter 2; Glucose Transporter GLUT2; Glucose transporter type 2; Glucose transporter type 2 liver; GLUT-2; GLUT2; GLUT 2; GTR2_HUMAN; SLC2A2; Solute carrier family 2 (facilitated glucose transporter) member 2; Solute carrier family 2 f

Cat:

SL0351R

Species Reactivity：

Human,Mouse,Rat,Goat,(predicted: Chicken,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human GLUT2:431-524/524<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ugLane 2: Pancreas (Mouse) Lysate at 40 ugPrimary: Anti-GLUT2 (SL0351R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50-53 kDObserved band size: 50 kDSample: embyo (Mouse) Lysate at 40 ugPrimary: Anti-GLUT2 (SL0351R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample: HT-29 Cell (Human) Lysate at 30 ugPrimary: Anti-GLUT2 (SL0351R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 59 kDSample:Placenta (Mouse)Lysate at 40 ugPrimary: Anti-GLUT2 (SL0351R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 54kDObserved band size: 54kDParaformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glut2) Polyclonal Antibody, Unconjugated (SL0351R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): Hep G2(blue). Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (SL0351R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Glucose transporter 2 isoform is an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. It mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. [provided by RefSeq, Jul 2008].

Function:
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Liver, insulin-producing beta cell, small intestine and kidney.

Post-translational modifications:
N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.

DISEASE:
Defects in SLC2A2 are the cause of Fanconi-Bickel syndrome (FBS) [MIM:227810]. FBS is a rare, well-defined clinical entity, inherited in an autosomal recessive mode and characterized by hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose.

Similarity:
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

SWISS:
P11168

Gene ID:
6514

Database links:

Entrez Gene: 6514 Human

Entrez Gene: 20526 Mouse

Entrez Gene: 25351 Rat

Omim: 138160 Human

SwissProt: P11168 Human

SwissProt: P14246 Mouse

SwissProt: P12336 Rat

Unigene: 167584 Human

Unigene: 18443 Mouse

Unigene: 89295 Rat

交换和转运（Trafficking and Transport）
葡萄糖载运蛋白2 GLUT-2是胰岛B细胞膜上的转运蛋白，在血糖浓度升高时，促进GLUT2对葡萄糖的转运功能，继而刺激胰岛素释放。GLUT2分子对葡萄糖亲合力极低，似乎仅在血浆葡萄糖水平相对较高时才作为转运体发挥载体功能,GLUT2在胰岛B细胞的葡萄糖转运中起着重要作用
GLUT-2和GLUT-4蛋白这两个葡萄糖运载体的研究对于糖尿病的胰岛素释放障碍和胰岛素抵抗有重要意义 Picture

Sample:
embyo (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (SL0351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample:
HT-29 Cell (Human) Lysate at 30 ug
Primary: Anti-GLUT2 (SL0351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 59 kD

Sample:Placenta (Mouse)Lysate at 40 ug
Primary: Anti-GLUT2 (SL0351R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 54kD

Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glut2) Polyclonal Antibody, Unconjugated (SL0351R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control (blue line): Hep G2(blue).
Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (SL0351R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (10) | SL0351R has been referenced in 10 publications.

[IF=2.59] Tian C et al. Short communication: A high-grain diet entails alteration in nutrient chemosensing of the rumen epithelium in goats. Animal Feed Science and Technology. Volume 262, April 2020, 114410. IHSLCP ;

PubMed:doi:10.1016/j.anifeedsci.2020.114410

[IF=3.871] Pengli Wang. et al. The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver. Mol Cell Endocrinol. 2020 Dec;518:111022 WB,IHC ; Rat.

PubMed:32871226

[IF=3.457] Wang N et al. Fibroblast growth factor 21 improves glucose homeostasis partially via down-regulation of Na+-d-glucose cotransporter SGLT1 in the small intestine.(2019) Biomedicine & Pharmacotherapy, 109, 1070–1077. WB ; Mouse.

PubMed:30551357

[IF=0] Song B et al. The effects of emodin on insulin resistance in KKAy mice with diabetes mellitus. Phcog Mag. 2018;14:344-50. IHSLCP ; Mouse.

PubMed:10.4103/pm.pm_362_17

[IF=4.81] Justice,et al.Inhibition of acid sphingomyelinase disrupts LYNUS signaling and triggers autophagy.(2018) Journal of Lipid Research. 59:596-606. IF(ICC) ; Human.

PubMed:29378782

[IF=3.144] Wang,et al.Oat globulin peptides regulate antidiabetic drug targets and glucose transporters in Caco-2 cells.(2018) Journal of Functional Foods. 42:12-20. WB ; Human.

PubMed:10.1016/j.jff.2017.12.061

[IF=3.358] Guttman et al. α1-Antitrypsin modifies general NK cell interactions with dendritic cells and specific interactions with islet β-cells in favor of protection from autoimmune diabetes. (2014) Immunology. FC/FACS ; Mouse.

PubMed:25308894

[IF=5.23] Zheng, Liming, et al. "The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation." Scientific Reports 6 (2016): 37414. IF(IHSLCP) ; Goat.

PubMed:27857213

[IF=1.813] Wang Zhe. et al. Total flavonoids of Astragalus Ameliorated Bile Acid Metabolism Dysfunction in Diabetes Mellitus. Evid-Based Compl Alt. 2021;2021:6675567 WB,IF ; Human, Mouse.

PubMed:33953787

[IF=3.571] Jiang T et al. Protein-bound Anthocyanin Compounds of Purple Sweet Potato (p-BASLCPSP) Ameliorate Hyperglycemia by Regulating Hepatic Glucose Metabolism in High Fat Diet/Streptozotocin-Induced Diabetic Mice. J Agric Food Chem. 2020 Feb 12;68(6):1596-1608. WB ; Mouse.

PubMed:31927925