Rabbit Anti-Caspase-12 antibody

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UNQ9415; Apoptosis related cysteine protease; CASP 12; casp12; CASP12P1; Caspase 12 pseudogene 1; CASP-12; casp12; caspase 12 (gene/pseudogene); Caspase12; CASPC_HUMAN; OTTHUMP00000207026; OTTHUMP00000207028; OTTHUMP00000207031; OTTHUMP00000207032; OTTHUM

Cat:

SL1105R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from the middle of human Caspase-12:201-300/420

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-alpha smooth muscle Actin (SL1105R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 46 kDObserved band size: 50 kDSample: Lane 1: Muscle (Mouse) Tissue Lysate at 40 ugLane 2: Muscle (Rat) Tissue Lysate at 40 ugPrimary: Anti-Caspase-12 (SL1105R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 46 kDObserved band size: 47/40 kDSample: Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Heart (Mouse) Lysate at 40 ugLane 3: Kidney (Mouse) Lysate at 40 ugPrimary: Anti-Caspase-12 (SL1105R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45/52 kDObserved band size: 46 kDParaformaldehyde-fixed, paraffin embedded (mouse fallopian tube); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (SL1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (SL1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Caspase-12 Polyclonal Antibody, Unconjugated(SL1105R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat testis tissue(high fat); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(SLR) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Caspases are cysteine proteases that cleave SLCterminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011].

Function:
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.

Tissue Specificity:
Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.

Similarity:
Belongs to the peptidase C14A family.
Contains 1 CARD domain.

SWISS:
O08736

Gene ID:
100506742

Database links:

Entrez Gene: 100506742 Human

Entrez Gene: 12364 Mouse

Entrez Gene: 156117 Rat

SwissProt: Q6UXS9 Human

SwissProt: O08736 Mouse

SwissProt: Q920D5 Rat

Omim: 608633 Human

Unigene: 476989 Human

Unigene: 81078 Rat

Caspase-12是caspase家族成员，是存在于内质网上的促凋亡因子，内质网应激早期可通过GRP（glucose-regulated protein，GRP）的表达增多来保护细胞；CASPASE-12是内质网细胞关键介导因子，caspase-12还是caspase-1的抑制剂。 Picture

Sample:
Lane 1: Muscle (Mouse) Tissue Lysate at 40 ug
Lane 2: Muscle (Rat) Tissue Lysate at 40 ug
Primary: Anti-Caspase-12 (SL1105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 47/40 kD

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-12 (SL1105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/52 kD
Observed band size: 46 kD

Paraformaldehyde-fixed, paraffin embedded (mouse fallopian tube); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (SL1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (SL1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-12 Polyclonal Antibody, Unconjugated(SL1105R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat testis tissue(high fat); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(SLR) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall

Specific References (17) | SL1105R has been referenced in 17 publications.

[IF=5.396] Yujie Zhong. et al. Dioscin relieves diabetic nephropathy via suppressing oxidative stress, apoptosis, and improving mitochondrial quality and quantity control. Food Funct. 2022 Feb;: WB ; Rat.

PubMed:35262539

[IF=6.284] Yujie Zhong. et al. Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca 2+ homeostasis. 2021 Mar 02 WB ; Rat.

PubMed:33651226

[IF=4.096] Xiaowei Sun. et al. Acupuncture protects against cerebral ischemia–reperfusion injury via suppressing endoplasmic reticulum stress-mediated autophagy and apoptosis. Mol Med. 2020 Dec;26(1):1-14 WB ; Rat.

PubMed:33167857

[IF=0.504] Zhongkui Li. et al. Down-modulation of endoplasmic reticulum stressinitiated apoptosis by huperzine A in isoproterenolprovoked myocardial infarction rat model: Role of Nrf2/HO- 1 signaling axis. Trop J Pharm Res. 2020 Nov;19(8):1685-1691 IHC ; Rat.

PubMed:10.4314/tjpr.v19i8.17

[IF=1.798] Wei-Ting Xuanet al. Berberine ameliorates rats model of combined Alzheimer’s disease and type 2 diabetes mellitus via the suppression of endoplasmic reticulum stress. 3 Biotech . 2020 Aug;10(8):359. WB ; rat.

PubMed:32832321

[IF=3.448] Sun Y et al. Effect of endoplasmic reticulum stress-mediated excessive autophagy on apoptosis and formation of kidney stones. Life Sci. 2019 Dec 26:117232. WB ; Rat.

PubMed:31816897

[IF=4.784] Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26. IHSLCP ; Mouse.

PubMed:30605745

[IF=4.65] Yu, H., et al. "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro."Cellular Physiology and Biochemistry 39.1 (2016): 123-136. WB ; Rat.

PubMed:27322831

[IF=1.88] Zhou, Yunchuan, et al. "Sulfiredoxin-1 exerts anti-apoptotic and neuroprotective effects against oxidative stress-induced injury in rat cortical astrocytes following exposure to oxygen-glucose deprivation and hydrogen peroxide."International Journal of Molecular Medicine (2015). WB ; Rat.

PubMed:25955519

[IF=2.2] Zhang, Huiying, et al. "Glucose-regulated protein 78 may play a crucial role in promoting the pulmonary microvascular remodeling in a rat model of hepatopulmonary syndrome." Gene (2014). IHSLCP ; Rat.

PubMed:24768185

[IF=6.023] Yujie Zhong. et al. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy. Food Chem Toxicol. 2022 Jan;159:112697 WB ; Rat.

PubMed:34826549

[IF=1.665] Rui Zhang. et al. Apigetrin ameliorates streptozotocin-induced pancreatic β-cell damages via attenuating endoplasmic reticulum stress. In Vitro Cell Dev-An. 2020 Sep;56(8):622-634 WB ; Rat.

PubMed:32901429

[IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551 WB ; Mouse.

PubMed:32996162

[IF=2.197] Luo Q et al. Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in PolyphyllinI-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells. Biomed Res Int. 2018 Dec 25;2018:5241941. WB ; Human.

PubMed:30671458

[IF=3.23] Wang, Yu, et al. "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." PloS one 12.4 (2017): e0173469. WB ; Rat.

PubMed:28399139

[IF=2.29] Wang, Yu, et al. "Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways." Heart and Vessels (2016): 1-8. WB ; Rat.

PubMed:27639990

[IF=1.89] Zhang, Lili, et al. "Increased expression of 78 kD glucose-regulated protein promotes cardiomyocyte apoptosis in a rat model of liver cirrhosis." International Journal of Clinical and Experimental Pathology 8.8 (2015): 9256-9263. IHSLCP ; Rat.

PubMed:26464674