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Home > Product > Antibody > Rabbit Anti-GAD65 antibody
65 kDa glutamic acid decarboxylase; DCE 2; DCE2; GAD 2; GAD 65; GAD-2; GAD-65; GAD2; Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa); Glutamate Decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Aci
Cat:
SL0325R
Species Reactivity：
Human,Mouse,Rat,(predicted: Chicken,Pig,)
Immunogen：
KLH conjugated synthetic peptide derived from human GAD65:501-585/585
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Hela Cell Lysate at 40 ugPrimary: Anti- GAD65 (SL0325R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilutionPredicted band size: 65 kDObserved band size: 65 kDSample: Lung(Rat) lysate at 30 ug; Primary: Anti-GAD65 (SL0325R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 65kDObserved band size : 65kDSample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Mouse Hippocampus tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Cerebellum tissue lysates Lane 6: Rat Hippocampus tissue lysates Lane 7: Human SH-SY5Y cell lysates Lane 8: Human U-87 MG cell lysates Lane 9: Human Hela cell lysates Lane 10: Human Jurkat cell lysates Primary: Anti-GAD65 (SL0325R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDaObserved band size: 62 kDaSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-GAD65 (SL0325R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDObserved band size: 65 kDSample: brain (Mouse) Lysate at 40 ugPrimary: Anti-GAD65 (SL0325R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDObserved band size: 65 kDTissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GAD65 Polyclonal Antibody, Unconjugated(SL0325R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAD65) Polyclonal Antibody, Unconjugated (SL0325R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product PDFs
Datasheet:


This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Function:
Catalyzes the production of GABA.

Subunit:
Homodimer.

Subcellular Location:
Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.

Post-translational modifications:
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.

Similarity:
Belongs to the group II decarboxylase family.

SWISS:
Q05329

Gene ID:
2572

Database links:

Entrez Gene: 2572 Human

Entrez Gene: 14417 Mouse

Entrez Gene: 2876 Rat

SwissProt: Q05329 Human

SwissProt: P4864 Mouse

SwissProt: Q05683 Rat

Unigene: 231829 Human

Unigene: 4784 Mouse

Unigene: 29951 Rat



谷氨酸脱羧酶-65(GAD65)是用于I II型糖尿病研究的很重要的蛋白。 Picture

Sample: Hela Cell Lysate at 40 ug
Primary: Anti- GAD65 (SL0325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample: Lung(Rat) lysate at 30 ug;
Primary: Anti-GAD65 (SL0325R) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size : 65kD
Observed band size : 65kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Mouse Hippocampus tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Cerebellum tissue lysates
Lane 6: Rat Hippocampus tissue lysates
Lane 7: Human SH-SY5Y cell lysates
Lane 8: Human U-87 MG cell lysates
Lane 9: Human Hela cell lysates
Lane 10: Human Jurkat cell lysates
Primary: Anti-GAD65 (SL0325R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 62 kDa
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GAD65 (SL0325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample:
brain (Mouse) Lysate at 40 ug
Primary: Anti-GAD65 (SL0325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GAD65 Polyclonal Antibody, Unconjugated(SL0325R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAD65) Polyclonal Antibody, Unconjugated (SL0325R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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