Rabbit Anti-Growth hormone receptor antibody

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GH receptor; GHBP; GHR; Growth hormone receptor precursor; Serum binding protein; Somatotropin receptor; GHR_HUMAN.

Cat:

SL0654R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human GHR:101-200/451<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Testis (Mouse) Lysate at 40 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: Testis (Rat) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-Growth hormone receptor (SL0654R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130/110 kDObserved band size: 130 kDThis image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (SL0654R) at 1:300 followed by conjugation to a secondary antibody and staining.Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GHR Polyclonal Antibody, Unconjugated(SL0654R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) .Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).Positive control: A549 cellsConcebtration: 1μg/10^6 cellsIncubation conditions: Avoid light , 30 minutes on the ice.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011].

Function:
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.
The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.

Subunit:
On growth hormone (GH) binding, forms homodimers and binds JAK2 via a box 1-containing domain. Binding to SOCS3 inhibits JAK2 activation, binding to CIS and SOCS2 inhibits STAT5 activation. Interacts with ADAM17.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway.
Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=Remains fixed to the cell membrane and is not internalized.
Growth hormone-binding protein: Secreted. Note=Complexed to a substantial fraction of circulating GH.

Tissue Specificity:
Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.

Post-translational modifications:
The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17.
On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization.

DISEASE:
Laron syndrome (LARS) [MIM:262500]: A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Note=The disease is caused by mutations affecting the gene represented in this entry.
Idiopathic short stature autosomal (ISSA) [MIM:604271]: Short stature is defined by a subnormal rate of growth. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 1 fibronectin type-III domain.

SWISS:
P10912

Gene ID:
2690

Database links:

Entrez Gene: 2690 Human

Entrez Gene: 1920 Mouse

Entrez Gene: 25235 Rat

Omim: 600946 Human

SwissProt: P10912 Human

SwissProt: P16882 Mouse

SwissProt: P16310 Rat

Unigene: 12536 Human

Unigene: 684631 Human

Unigene: 3986 Mouse

Unigene: 2178 Rat

生长激素受体(growth hormone receptor，GHR)是细胞因子/造血因子受体超级家族成员之一。
生长激素受体通过二聚体的形式和生长激素(growth hormone, GH)相结合，然后诱发Janus 激酶2 (Janus kinase 2，JAK2：是很多细胞因子、生长因子及干扰素的重要信号传感器)等细胞因子酪氨酸磷酸化并通过4条不同的途径将信号传入细胞内从而产生一系列的生理效应。 Picture

This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (SL0654R) at 1:300 followed by conjugation to a secondary antibody and staining.

Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GHR Polyclonal Antibody, Unconjugated(SL0654R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) .
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).

Positive control: A549 cells
Concebtration: 1μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.

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Specific References (10) | SL0654R has been referenced in 10 publications.

[IF=0.86] Zhou et al. Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets. (2018) Asian-Australas.J.Anim.Sci. 31:32-39 IHSLCP ; Pig.

PubMed:28920404

[IF=2.23] Chen et al. Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis. (2013) Neural.Regen.Res. 8:1756-64 WB ; Rat.

PubMed:25206472

[IF=3.86] Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017). WB ; Human.

PubMed:28599789

[IF=1.84] Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016). WB ; Sheep.

PubMed:28043364

[IF=4.24] Hetz, Jennifer A., et al. "Growth axis maturation is linked to nutrition, growth and developmental rate." Molecular and Cellular Endocrinology (2015). WB ;

PubMed:25896544

[IF=2.02] Wang, Zheng, et al. "RhGH attenuates ischemia injury of intrahepatic bile ducts relating to liver transplantation." Journal of Surgical Research 171.1 (2011): 300-310. IHSLCP ; Rat.

PubMed:20462597

[IF=2.51] Yang, Hai Li, et al. "Effect of suppressor of cytokine signaling 2 (SOCS2) on fat metabolism induced by growth hormone (GH) in porcine primary adipocyte." Molecular biology reports 39.9 (2012): 9113-9122. WB ; Pig.

PubMed:22729878

[IF=2.445] Xie W et al. Seasonal expressions of growth hormone receptor, insulin-like growth factor 1 and insulin-likegrowth factor 1 receptor in the scented glands of the muskrats (Ondatra zibethicus). Gen Comp Endocrinol. 2019 May 20;281:58-66. IHSLCP&WB ; muskrat.

PubMed:31121166

[IF=4.12] Cui et al. Generation of a miniature pig disease model for human Laron syndrome. (2015) Sci.Rep. 5:15603 WB ; Porcine.

PubMed:26511035

[IF=1.27] Song, Cheng-Jun, et al. "Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes." International Journal of Clinical and Experimental Medicine 8.7 (2015): 10411-10419. WB ; Rat.

PubMed:26379831