Rabbit Anti-GLUT4 antibody | Sunlong Medical

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insulin-responsive; Glucose transporter GLUT 4; Glucose Transporter GLUT4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GTR4_HUMAN; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporte

Cat:

SL0384R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human GLUT4:401-509/509<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart(Rat) Cell Lysate at 40 ugPrimary: Anti-GLUT4 (SL0384R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- GLUT4 (SL0384R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 2: Heart (Mouse) Lysate at 40 ugLane 3: Heart (Rat) Lysate at 40 ugLane 4: Muscle (Mouse) Lysate at 40 ugLane 5: Muscle (Rat) Lysate at 40 ugPrimary: Anti-GLUT4 (SL0384R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kDTissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(SL0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(SL0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (SL0384R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (blue line): K562 (blue). Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (SL0384R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 70% ethanol (overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.

Function:
Insulin-regulated facilitative glucose transporter.

Subcellular Location:
Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.

Tissue Specificity:
Skeletal and cardiac muscles; brown and white fat.

Post-translational modifications:
Sumoylated.

DISEASE:
Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects.

Similarity:
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

SWISS:
P14672

Gene ID:
6517

Database links:

Entrez Gene: 282359 Cow

Entrez Gene: 6517 Human

Entrez Gene: 20528 Mouse

Entrez Gene: 25139 Rat

Omim: 138190 Human

SwissProt: Q27994 Cow

SwissProt: Q29RP5 Cow

SwissProt: P14672 Human

SwissProt: P14142 Mouse

SwissProt: P19357 Rat

Unigene: 380691 Human

Unigene: 10661 Mouse

Unigene: 1314 Rat

葡萄糖转运蛋白-4是一种十分重要的葡萄糖转运体,与胰岛素抵抗和2型糖尿病密切相关.葡萄糖转运蛋白4在细胞内部和细胞膜之间循环流动.实现对葡萄糖的转运需要葡萄糖转运蛋白4自身的转位和活化.葡萄糖转运蛋白4与肥胖、肿瘤相关联.
GLUT-2和GLUT-4蛋白这两个葡萄糖运载体的研究对于糖尿病的胰岛素释放障碍和胰岛素抵抗有重要意义. Picture

Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- GLUT4 (SL0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Lane 4: Muscle (Mouse) Lysate at 40 ug
Lane 5: Muscle (Rat) Lysate at 40 ug
Primary: Anti-GLUT4 (SL0384R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(SL0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (SL0384R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control (blue line): K562 (blue).
Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (SL0384R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (18) | SL0384R has been referenced in 18 publications.

[IF=4.362] WB, IHC ; Mouse.

PubMed:32973490

[IF=1.88] Shihe Zhang. et al. Astragalus polysaccharide regulates brown adipocytes differentiation by miR-6911 targeting Prdm16. 2021 Nov 05 WB ; Mouse.

PubMed:34738642

[IF=5.279] Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB ; Mouse.

PubMed:34706542

[IF=2.629] Cao Zhanhong. et al. Protective Effects of Huangqi Shengmai Yin on Type 1 Diabetes-Induced Cardiomyopathy by Improving Myocardial Lipid Metabolism. Evid-Based Compl Alt. 2021;2021:5590623 WB ; Mouse.

PubMed:34249132

[IF=2.146] Xiaolan Wang. et al. The total flavonoids from Selaginella tamariscina (beauv.) Spring improve glucose and lipid metabolism in db/db mice. Iran J Basic Med Sci. 2020 Oct; 23(10): 1286–1292 WB ; Rat.

PubMed:33149860

[IF=4.362] Zhang Yan-hui. et al. α-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1α Signaling Pathway in P301S Mice. Front Aging Neurosci. 2020 Aug;12:262 WB ; Mouse.

PubMed:32973490

[IF=2.659] Ye X et al. Irisin reverses insulin resistance in C2C12 cells via the p38-MAPK-PGSLC1α pathway. Peptides. 2019 Jul 24:170120. WB ; Mouse.

PubMed:31351089

[IF=0] Song B et al. The effects of emodin on insulin resistance in KKAy mice with diabetes mellitus. Phcog Mag. 2018;14:344-50. IHSLCP ; Mouse.

PubMed:10.4103/pm.pm_362_17

[IF=5.5] Sikder et al. High Fat Diet Upregulates Fatty Acid Oxidation and Ketogenesis via Intervention of PPAR-γ. (2018) Cell.Physiol.Biochem. 48:1317-1331 WB ; Mouse.

PubMed:30048968

[IF=1.58] Gao, Sujie, et al. "Propofol inhibits growth of neurons through regulating insulin receptor and insulin-like growth factor-1 receptor." Int J Clin Exp Pathol 9.7 (2016): 6785-6794. WB ; Rat.

PubMed:ISSN:1936-2625/IJCEP0023819

[IF=3.3] Zhang, Shihai, et al. "Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2." British Journal of Nutrition (2016): 1-10. WB ; Mouse, Pig.

PubMed:27464458

PubMed:34706542

[IF=3.871] Pengli Wang. et al. The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver. Mol Cell Endocrinol. 2020 Dec;518:111022 WB,IHC ; Rat.

PubMed:32871226

[IF=3.998] Liza D. Morales. et al. Further evidence supporting a potential role for ADH1B in obesity. Sci Rep-Uk. 2021 Jan;11(1):1-14 WB ; Human.

PubMed:33479282

[IF=3.69] Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947 WB ; Rat.

PubMed:33617969

[IF=1.984] Pan LL et al. Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model. Evidence-Based Complementary and Alternative Medicine, 2019, 1–17. WB ; Rat.

PubMed:doi:10.1155/2019/1394641

[IF=2.302] Li Q et al. All-trans retinoic acid regulates sheep primary myoblasts proliferation and differentiation in vitro. Domestic Animal Endocrinology,2019 106394. WB ; Sheep.

PubMed:doi:10.1016/j.domaniend.2019.106394

[IF=1.832] Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193. WB ; ram lambs.

PubMed:31022943