Rabbit Anti-CHRNA7 antibody

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CHRFAM7A; ACHA7_HUMAN; cholinergic receptor, nicotinic, alpha 7; Neuronal acetylcholine receptor subunit alpha-7; ACHR ALPHA 7; AChR alpha 7 Receptor; Acra7; ALPHA-7NACHR; ALPHA7; ALPHA7 NICOTINIC ACETYLCHOLINE RECEPTOR; Alpha7 nicr; BTX; CHRNA7; CHRNA7-2

Cat:

SL1049R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,)

Immunogen：

KLH conjugated synthetic peptide derived from human CHRNA7:441-502/502<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human SH-SY5Y cell Lysates Lane 2: Human U-87 MG cell Lysates Lane 3: Human U251 cell Lysates Primary: Anti-CHRNA7 (SL1049R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55kDaObserved band size: 55kDaSample: Lane 1: Cerebrum (Rat) Lysate at 40 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: Adrenal glands (Rat) Lysate at 40 ugLane 4: Adrenal glands (Mouse) Lysate at 40 ugLane 5: Placenta (Mouse) Lysate at 40 ugLane 6: Lung (Mouse) Lysate at 40 ugLane 7: Testis (Rat) Lysate at 40 ugLane 8: HepG2 (Human) Cell Lysate at 30 ugLane 9: SH-SY5Y (Human) Cell Lysate at 30 ugPrimary: Anti-CHRNA7 (SL1049R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56’50 kDObserved band size: 48 kDSample: Cerebrum (Mouse) Lysate at 40 ugPlacenta (Mouse) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugThymus (Mouse) Lysate at 40 ugLymph node (Mouse) Lysate at 40 ugPrimary: Anti- CHRNA7 (SL1049R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 53 kDParaformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (SL1049R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(SL1049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(SL1049R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Nicotinic Acetylcholine Receptors are members of a superfamily of ligand gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be hetero pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C terminal extracellular region. The Nicotinic Acetylcholine Receptor alpha 7 forms a homo oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.

Function:
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

Subunit:
Homopentamer. Interacts with RIC3; which is required for proper folding and assembly.

Subcellular Location:
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.

SWISS:
P36544

Gene ID:
1139

Database links:

Entrez Gene: 1139 Human

Entrez Gene: 374001 Chicken

Entrez Gene: 282178 Cow

Entrez Gene: 11441 Mouse

Entrez Gene: 25302 Rat

Omim: 118511 Human

SwissProt: P22770 Chicken

SwissProt: P54131 Cow

SwissProt: P36544 Human

SwissProt: Q8IUZ4 Human

SwissProt: P49582 Mouse

SwissProt: Q05941 Rat

Unigene: 88 Cow

Unigene: 511772 Human

Unigene: 113464 Mouse

Unigene: 9698 Rat

Picture

Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Adrenal glands (Rat) Lysate at 40 ug
Lane 4: Adrenal glands (Mouse) Lysate at 40 ug
Lane 5: Placenta (Mouse) Lysate at 40 ug
Lane 6: Lung (Mouse) Lysate at 40 ug
Lane 7: Testis (Rat) Lysate at 40 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Lane 9: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-CHRNA7 (SL1049R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56’50 kD
Observed band size: 48 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Placenta (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- CHRNA7 (SL1049R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (SL1049R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(SL1049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(SL1049R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Specific References (8) | SL1049R has been referenced in 8 publications.

[IF=4.711] Martins I. C. A.. et al. Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the α7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response. Mediat Inflamm. 2021;2021:5526940 WB,IF ; Mouse.

PubMed:34421366

[IF=3.263] Mahmoud E. Youssef. et al. α7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996 IHC ; Rat.

PubMed:33684450

[IF=2.172] Marta Pernarella. et al. Effects mediated by the α7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells. Eur J Histochem. 2020 Jun 19; 64(Suppl 2): 3159 WB,IHC ; Rat.

PubMed:33334089

[IF=15.84] Han Yang. et al. A Novel Targeted and High‐Efficiency Nanosystem for Combinational Therapy for Alzheimer's Disease. Adv Sci. 2020 Oct;7(19):1902906 ELISA,FC ; Mouse.

PubMed:33042734

[IF=3.212] Yang Gao. et al. Mechanisms of Renal-Splenic Axis Involvement in Acute Kidney Injury Mediated by the α7nAChR-NF-κB Signaling Pathway. 2020 Nov 03 WB ; Mouse.

PubMed:33141376

[IF=4.367] Sallam MY et al. Brainstem cholinergic pathways diminish cardiovascular and neuroinflammatory actions of endotoxemia in rats: Role of NFκB/α7/α4β2AChRs signaling. Neuropharmacology. 2019 Jun 25;157:107683. IHSLCP ; Rat.

PubMed:31247270

[IF=3.266] Gao Y et al. Effect of splenectomy on attenuation of LPS-induced AKI through GTS-21-induced cholinergic anti-inflammatory pathway. Am J Transl Res. 2019 Apr 15;11(4):2540-2549. WB ; Mouse.

PubMed:31105861

[IF=3.608] T.C. Peixoto. et al. Nicotine exposure during lactation causes disruption of hedonic eating behavior and alters dopaminergic system in adult female rats. Appetite. 2021 May;160:105115 WB ; Rat.

PubMed:33453337