Rabbit Anti-CEBP Beta antibody

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CEBP-beta; CLEBP beta; CCAAT Enhancer Binding Protein beta; AGP/EBP; C EBP beta; C/EBP related protein 2; CEBPB; CRP2; IL 6DBP; IL6DBP; Interleukin 6 dependent binding protein; LAP; Liver enriched transcriptional activator; NF IL6; NFIL6; Nuclear factor N

Cat:

SL1396R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human CEBP Beta:251-345/345

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1µg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Muscle (Mouse) Lysate at 40 ugPrimary: Anti-CEBP Beta (SL1396R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 41 kDSample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-CEBP Beta (SL1396R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample: MG63(Human) Cell Lysate at 40 ugPrimary: Anti-CEBP Beta (SL1396R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample: HepG2(Human) Cell Lysate at 40 ugPrimary: Anti-CEBP Beta (SL1396R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBP Beta) Polyclonal Antibody, Unconjugated (SL1396R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): Hela Primary Antibody (green line): Rabbit Anti-CEBP Beta antibody (SL1396R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq].

Function:
Involved in interleukin-6 signal transduction, including the transcriptional activation of acute-phase genes. Functions in brown adipose tissue (BAT) differentiation.

Subunit:
Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B.

Subcellular Location:
Nucleus.

Post-translational modifications:
Sumoylated by polymeric chains of SUMO2 or SUMO3.

Similarity:
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain.

SWISS:
P17676

Gene ID:
1051

Database links:

Entrez Gene: 1051 Human

Entrez Gene: 12608 Mouse

Entrez Gene: 24253 Rat

Omim: 189965 Human

SwissProt: P17676 Human

SwissProt: P28033 Mouse

SwissProt: P21272 Rat

Unigene: 517106 Human

Unigene: 719041 Human

Unigene: 720603 Human

Unigene: 439656 Mouse

Unigene: 6479 Rat

转录调节因子（Transcriptin Regulators） Picture

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-CEBP Beta (SL1396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Sample:
MG63(Human) Cell Lysate at 40 ug
Primary: Anti-CEBP Beta (SL1396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-CEBP Beta (SL1396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBP Beta) Polyclonal Antibody, Unconjugated (SL1396R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control (blue line): Hela
Primary Antibody (green line): Rabbit Anti-CEBP Beta antibody (SL1396R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (10) | SL1396R has been referenced in 10 publications.

[IF=1.88] Shihe Zhang. et al. Astragalus polysaccharide regulates brown adipocytes differentiation by miR-6911 targeting Prdm16. 2021 Nov 05 WB ; Mouse.

PubMed:34738642

[IF=3.105] Huan-Tong Wu. et al. Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice. Neurotoxicology. 2021 Jul;85:68 WB ; Rat.

PubMed:34004234

[IF=4.357] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human.

PubMed:26538209

[IF=5.62] He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015). WB ; Human.

PubMed:26272182

[IF=1.922] Wang F et al. Insulin‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Mol Med Rep. 2019 Jan 24. WB ; Human.

PubMed:30144075

[IF=4.575] Ren, Teng-Teng. et al. Gisenoside Rg1 attenuates cadmium-induced neurotoxicity in vitro and in vivo by attenuating oxidative stress and inflammation. Inflamm Res. 2021 Dec;70(10):1151-1164 WB ; Mouse.

PubMed:34661679

[IF=2.02] Jiejuan Lai. et al. Comparison of the biological and functional characteristics of mesenchymal stem cells from intrahepatic and identical bone marrow. Stem Cell Res. 2021 Aug;55:102477 IHC ; Mouse.

PubMed:34343826

[IF=4.259] Wu Z et al. Co-infection of Mycoplasma gallisepticum and Escherichia coli Triggers Inflammatory Injury Involving the IL-17 Signaling Pathway. Front Microbiol. 2019 Nov 15;10:2615. WB ; Chicken.

PubMed:3363158

[IF=1.832] Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193. WB ; ram lambs.

PubMed:31022943

[IF=1.448] Zhang K et al. Insulin‑like growth factor 2 promotes the adipogenesis of hemangioma‑derived stem cells. Exp Ther Med. 2019 Mar;17(3):1663-1669. WB ; Human.

PubMed:30867686