Rabbit Anti-Gremlin antibody

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CKTSF1B1; GREMLIN-1; GREMLIN 1; GREMLIN1; Cysteine knot superfamily BMP antagonist 1; DAND2; DRM; GREMLIN; Gremlin1; Gremlin 1; Gremlin-1; IHG2; PIG2; proliferation inducing gene 2 protein.

Cat:

SL1475R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human gremlin:101-184/184

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti- Gremlin (SL1475R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 21 kDSample: intestine (Mouse) Lysate at 40 ugskin (Mouse) Lysate at 40 ugPrimary: Anti-Gremlin (Bs-1475R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 24 kD Paraformaldehyde-fixed, paraffin embedded (rat rectum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (SL1475R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated(SL1475R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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In Xenopus, Gremlin 1 belongs to a novel gene family that act as BMP antagonists in embryonic explants. The human homolog of Gremlin 1(CKTFS1B1) is localized on human chromosome 15q13--> q15. Gremlin 1-specific mRNA is expressed in different human tissues, including brain, ovary, intestine and colon. It has been suggested that down-regulation of Gremlin 1 is associated with tumor progression, and support the hypothesis that human Gremlin 1 may play an important role during both neuroembryological development and carcinogenesis.

Function:
Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis.

Subunit:
Interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner.

Subcellular Location:
Secreted.

Tissue Specificity:
Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts.

Similarity:
Belongs to the DAN family.
Contains 1 CTCK (SLCterminal cystine knot-like) domain.

SWISS:
O60565

Gene ID:
26585

Database links:

Entrez Gene: 26585 Human

Entrez Gene: 23892 Mouse

Entrez Gene: 50566 Rat

Omim: 603054 Human

SwissProt: O60565 Human

SwissProt: O70326 Mouse

SwissProt: O35793 Rat

Unigene: 40098 Human

Unigene: 166318 Mouse

Unigene: 42929 Rat

gremlin属于骨形态形成蛋白BMP拮抗剂家族成员,在胚胎发育过程中可通过调节BMP活性，影响器官、体征的形成和组织分化。 Picture

Sample:
intestine (Mouse) Lysate at 40 ug
skin (Mouse) Lysate at 40 ug
Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 24 kD

Paraformaldehyde-fixed, paraffin embedded (rat rectum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (SL1475R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated(SL1475R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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