Rabbit Anti-SREBP1 antibody

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Sterol regulatory element binding protein 1; ADD 1; BHLHD1; D630008H06; SREBF 1; SREBP-1; SREBF1; SREBP 1; SREBP1; SREBP 1c; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulato

Cat:

SL1402R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human SREBP-1:301-450/1147

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7(Human) Cell Lysate at 30 ugA549(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugSH-SY5Y(Human) Cell Lysate at 30 ugU87MG(Human) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugPrimary: Anti-SREBP1 (SL1402R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 124/65 kDObserved band size: 124 kDParaformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCASLC3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCASLC3').

Subunit:
Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Processed sterol regulatory element-binding protein 1: Nucleus.
Isoform SREBP-1aDelta: Nucleus.
Isoform SREBP-1cDelta: Nucleus.

Tissue Specificity:
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Post-translational modifications:
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.

Similarity:
Belongs to the SREBP family.
Contains 1 bHLH (basic helix-loop-helix) domain.

SWISS:
P36956

Gene ID:
6720

Database links:

Entrez Gene: 6720 Human

Entrez Gene: 20787 Mouse

Entrez Gene: 78968 Rat

Omim: 184756 Human

SwissProt: P36956 Human

SwissProt: Q9WTN3 Mouse

SwissProt: P56720 Rat

Unigene: 592123 Human

Unigene: 278701 Mouse

Unigene: 296366 Mouse

Unigene: 198857 Rat

转录调节因子（Transcriptin Regulators）
胆固醇调节元件结合蛋白1(SREBP1)是膜结合的转录因子,参与脂质自稳态的许多作用(胆固醇、脂肪酸、甘油三酯和磷酯的合成/摄取).SREBP1在内质网上合成,需要被转运到高尔基体并经过一系列蛋白水解后才能到核内发挥作用。
未成熟时在胞浆表达，水解后的成熟片段在胞核表达。 Picture

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (SL1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Feedback Wall

Specific References (25) | SL1402R has been referenced in 25 publications.

[IF=2.72] Tiange Li. et al. Chitosan oligosaccharide attenuates hepatic steatosis in HepG2 cells via the activation of AMP-activated protein kinase. 2022 Feb 20 WB ; Human.

PubMed:10.1111/jfbc.14045

[IF=3.738] Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1α and β-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16 WB ; Bovine.

PubMed:35080710

[IF=9.225] Nora Helmrich. et al. Pharmacological Antagonization of Cannabinoid Receptor 1 Improves Cholestasis in Abcb4-/- Mice. Cell Mol Gastroenter. 2021 Dec;: WB ; Mouse.

PubMed:34954190

[IF=5.279] Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB ; Mouse.

PubMed:34706542

[IF=3.848] Chen Luyao. et al. Epicatechin gallate prevents the de novo synthesis of fatty acid and the migration of prostate cancer cells. Acta Bioch Bioph Sin. 2021 Oct;: WB ; Human.

PubMed:34718375

[IF=4.008] Flavia G De Carvalho. et al. Taurine upregulates insulin signaling and mitochondrial metabolism in vitro, but not in adipocytes of obese women. Nutrition. 2021 Jul;:111430 WB ; Mouse.

PubMed:10.1016/j.nut.2021.111430

[IF=4.171] Yunhong Ren. et al. Probiotic-fermented black tartary buckwheat alleviates hyperlipidemia and gut microbiota dysbiosis in rats fed with high-fat diet. 2021 May 05 WB ; Rat.

PubMed:34037655

[IF=1.487] Chengjian Zhou . et al. Daidzein stimulates fatty acid-induced fat deposition in C2C12 myoblast cells via the G protein-coupled receptor 30 pathway. 2020 Nov 08 WB ; Mouse.

PubMed:33164657

[IF=4.171] Chuang Guoet al. Recombinant Human lactoferrin attenuates the progression of hepatosteatosis and hepatocellular death by regulating iron and lipid homeostasis in ob/ob mice. Food Funct . 2020 Aug 1;11(8):7183-7196. WB ;

PubMed:32756704

[IF=3.647] Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci . 2020 Oct 1;258:118147. WB ; Human.

PubMed:32721464

[IF=3.571] Li X et al. Lysine enhances the stimulation of fatty acids on milk fat synthesis via the GPRC6A-PI3K-FABP5 signaling in bovine mammary epithelial cells. J Agric Food Chem. 2019 Jun 26;67(25):7005-7015. WB ; Cow.

PubMed:31174423

[IF=5.5] Fu et al. Lack of ClSLC2 Alleviates High Fat Diet-Induced Insulin Resistance and Non-Alcoholic Fatty Liver Disease. (2018) Cell.Physiol.Biochem. 45:2187-2198 WB ; Mouse.

PubMed:29550812

[IF=4.12] Ma et al. Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats. (2017) Sci.Rep. 7:44819 WB ; Rat.

PubMed:28300221

[IF=7.67] Zhang, Xian, et al. "ROS-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation."Antioxidants and Redox Signaling ja (2015). WB ; Rat.

PubMed:25602171

[IF=6.15] Hu, Shuang. et al. Deletion of p38γ attenuates ethanol consumption- and acetaminophen-induced liver injury in mice through promoting Dlg1. Acta Pharmacol Sin. 2021 Nov;:1-16 WB ; Mouse.

PubMed:34789918

[IF=2.534] Dongmin Zou. et al. SHBA regulates the expressions of lipid synthesis and oxidation genes in sheep hepatocytes through the AMPK pathway. Res Vet Sci. 2021 Nov;140:153 WB ; Sheep.

PubMed:34481206

[IF=5.717] Jia Han. et al. Zonarol Protected Liver from Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease in a Mouse Model. Nutrients. 2021 Oct;13(10):3455 WB ; Mouse.

PubMed:10.3390/nu13103455

[IF=4.225] Yuyan Gu. et al. Ferulic Acid Ameliorates Atherosclerotic Injury by Modulating Gut Microbiota and Lipid Metabolism. Front Pharmacol. 2021; 12: 621339 WB,IF ; Mouse.

PubMed:33841148

[IF=3.241] Sun W et al. Baicalein reduced hepatic fat accumulation by activating AMPK in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin resistant mice. Food Funct. 2020 Jan 7. WB ; Mouse.

PubMed:31909773

[IF=3.448] Yang X et al. miR‐760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c‐Myc. J Cell Biochem. 2019 Nov 10. WB ; Human.

PubMed:31709636

[IF=3.571] Li P et al. CRTC2 is a key mediator of amino acid-induced milk fat synthesis in mammary epithelial cells. J Agric Food Chem. 2019 Sep 18;67(37):10513-10520. WB ; Cow.

PubMed:31475823

[IF=3.234] Dong S et al. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sci. 2019 Jun 15;227:20-29. WB ; Mouse.

PubMed:30974116

[IF=2.705] Zhao Z et al. Cholesterol attenuated the progression of DEN-induced hepatocellular carcinoma via inhibiting SCAP mediated fatty acid de novo synthesis. Biochem Biophys Res Commun. 2019 Feb 19;509(4):855-861. WB ; Mouse.

PubMed:30638930

[IF=3.234] Vicente JM et al. The absence of lactation after pregnancy induces long-term lipid accumulation in maternal liver of mice.(2018) Life Sci. 217 WB ; Mouse.

PubMed:30562489

[IF=3.2] Guo, Xiao-fei, et al. "fat-1 mice protect against high-fat plus high-sugar diets induced non-alcoholic fatty liver disease." Food & Function (2017). WB ; Mouse.

PubMed:28972610