Rabbit Anti-phospho-STAT3 (Tyr705) antibody

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STAT3 (phospho Y705); p-STAT3 (phospho Y705); Phospho-Stat3（pTyr705）; STAT3(Phospho-Tyr705); p-STAT3(Tyr705); Phosphorylated Stat3(pTyr705); p-Stat3; Acute Phase Response Factor; APRF; DNA binding protein APRF; FLJ20882; MGC16063; Signal Transductor and

Cat:

SL1658R

Species Reactivity：

Human,Mouse,Rat,Chicken,(predicted: Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705:AP(p-Y)LK

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Heart (Mouse) Lysate at 40 ugLane 2: Kidney (Mouse) Lysate at 40 ugLane 3: Aorta (Rat) Lysate at 40 ugPrimary: Anti-phospho-STAT3 (Tyr705) (SL1658R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 92/84 kDObserved band size: 84 kDSample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti-phospho-STAT3 (Tyr705) (SL1658R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 85 kDObserved band size: 85 kDSample: Heart (Mouse) Lysate at 40 ugHeart (Rat) Lysate at 40 ugPrimary: Anti-p-STAT3 (SL1658R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 85 kDObserved band size: 85 kDParaformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human gastric cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti- phospho-STAT3 Polyclonal Antibody, Unconjugated(SL1658R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingParaformaldehyde-fixed, paraffin embedded (Transplantation tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phosphorylated signal Transductor and Activator of Transcription 3; p-STAT3) Polyclonal Antibody, Unconjugated (SL1658R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.This image has been kindly submitted by Tübingen Ageing and Tumour Immunology Group (TATI), Centre for Medical Research (ZMF), University of Tübingen Medical School www.tati-group.de via our distributor Biozol. Human Melanoma cell line, EST02, was stained with Rabbit Anti-phospho-STAT3 (Tyr705) Polyclonal Antibody, FITC Conjugated (SL1658R-FITC)for 30 minutes, on ice.Blank control: A431. Primary Antibody (green line): Rabbit Anti-phospho-STAT3 (Tyr705) antibody (SL1658R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Function:
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.

Subcellular Location:
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.

Tissue Specificity:
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Post-translational modifications:
Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.

DISEASE:
Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.

SWISS:
P40763

Gene ID:
6774

Database links:

Entrez Gene: 6774 Human

Entrez Gene: 20848 Mouse

Entrez Gene: 25125 Rat

Omim: 102582 Human

SwissProt: P40763 Human

SwissProt: P42227 Mouse

SwissProt: P52631 Rat

Unigene: 463059 Human

转录调节因子（Transcriptin Regulators） Picture

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-phospho-STAT3 (Tyr705) (SL1658R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Sample:
Heart (Mouse) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti-p-STAT3 (SL1658R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (SL1658R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human gastric cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti- phospho-STAT3 Polyclonal Antibody, Unconjugated(SL1658R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Paraformaldehyde-fixed, paraffin embedded (Transplantation tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phosphorylated signal Transductor and Activator of Transcription 3; p-STAT3) Polyclonal Antibody, Unconjugated (SL1658R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

This image has been kindly submitted by Tübingen Ageing and Tumour Immunology Group (TATI), Centre for Medical Research (ZMF), University of Tübingen Medical School www.tati-group.de via our distributor Biozol.
Human Melanoma cell line, EST02, was stained with Rabbit Anti-phospho-STAT3 (Tyr705) Polyclonal Antibody, FITC Conjugated (SL1658R-FITC)for 30 minutes, on ice.

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-phospho-STAT3 (Tyr705) antibody (SL1658R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (28) | SL1658R has been referenced in 28 publications.

[IF=1.066] Zong-Ding Wang. et al. A Novel Hepatectomy Model in Mice Using a Gutta Cutter Tool: A Feasibility Study and Preliminary Results. Transpl P. 2022 Mar;: IHC ; Mouse.

PubMed:35287968

[IF=5.279] Yan Zhang. et al. Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimer’s Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis. J Agr Food Chem. 2021;69(47):14204–14214 WB ; Mouse.

PubMed:34798773

[IF=5.037] Hongwu Meng. et al. Interleukin-9 attenuates inflammatory response and hepatocyte apoptosis in alcoholic liver injury. Life Sci. 2022 Jan;288:12036 WB ; Mouse.

PubMed:34843736

[IF=4.105] Ai Jin. et al. TSLP-induced collagen type-I synthesis through STAT3 and PRMT1 is sensitive to calcitriol in human lung fibroblasts. Bba-Mol Cell Res. 2021 Jun;:119083 WB ; Human.

PubMed:34147561

[IF=2.886] Yusi Liu. et al. Synergistic Effects of Resveratrol and Temozolomide Against Glioblastoma Cells: Underlying Mechanism and Therapeutic Implications. Cancer Manag Res. 2020; 12: 8341–8354 ICC ; Rat, Human.

PubMed:32982428

[IF=2.05] Zhang M et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachloride–induced liver fibrosis in mice. Naunyn Schmiedebergs Arch Pharmacol . 2020 May 30. WB ; Mouse.

PubMed:32474674

[IF=2.736] Hao Y et al. IL-6/STAT3 mediates the HPV18 E6/E7 stimulated upregulation of MALAT1 gene in cervical cancer HeLa cells. Virus Res. 2020 May;281:197907. WB ; human.

PubMed:3222834

[IF=4.868] Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324. WB ; Mouse.

PubMed:31182999

[IF=3.414] Zhou H et al. Paris saponin VII extracted from trillium tschonoskii suppresses proliferation and induces apoptosis of human colorectal cancer cells. J Ethnopharmacol. 2019 Jul 15;239:111903. WB ; Human.

PubMed:31047966

[IF=2.81] Badin et al. Muscle Arnt/Hif1β Is Dispensable in Myofiber Type Determination, Vascularization and Insulin Sensitivity. (2016) PLoS.One. 11:e0168457 WB ; Mouse.

PubMed:28005939

[IF=3.36] Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234 FC/FACS ; Human.

PubMed:27726309

[IF=2.82] Gao, Ling, et al. "Morusin shows potent antitumor activity for human hepatocellular carcinoma in vitro and in vivo through apoptosis induction and angiogenesis inhibition." Drug Design, Development and Therapy 11 (2017): 1789. WB ; Human.

PubMed:28670112

[IF=2.991] Yang L et al. 15-Lipoxygenase-2/15(S)-hydroxyeicosatetraenoic acid regulates cell proliferation and metastasis via the STAT3 pathway in lung adenocarcinoma. Prostaglandins Other Lipid Mediat. 2018 Sep;138:31-40. WB&IHSLCP ; Human.

PubMed:30110652

[IF=2.705] Li B et al. Increased hepcidin in hemorrhagic plaques correlates with iron-stimulated IL-6/STAT3 pathway activation in macrophages. Biochem Biophys Res Commun. 2019 Jul 23;515(2):394-400. IHC,WB ; Rabbit,Human&Mouse.

PubMed:31153641

[IF=3.269] Leiming Sun. et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered. 2021;12(2):11188-1240 WB ; Rat.

PubMed:34784849

[IF=3.347] Yuying Li. et al. Anthraquinone derivative C10 inhibits proliferation and cell cycle progression in colon cancer cells via the Jak2/Stat3 signaling pathway. Toxicol Appl Pharm. 2021 Mar;:115481 IF ; Human.

PubMed:33722666

[IF=4.187] Jiajia Wang. et al. TAK-242 ameliorates DSS-induced colitis by regulating the gut microbiota and the JAK2/STAT3 signaling pathway. Microb Cell Fact. 2020 Dec;19(1):1-17 WB ; Mouse.

PubMed:32762699

[IF=2.05] Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachloride–induced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204 WB ; Mouse.

PubMed:32474674

[IF=3.606] Xuejiao Zhou. et al. The novel ALK inhibitor ZX‐29 induces apoptosis through inhibiting ALK and inducing ROS‐mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02 WB ; Human.

PubMed:33140567

[IF=2.51] Yang, Hai Li, et al. "Effect of suppressor of cytokine signaling 2 (SOCS2) on fat metabolism induced by growth hormone (GH) in porcine primary adipocyte." Molecular biology reports 39.9 (2012): 9113-9122. WB ; Pig.

PubMed:22729878

[IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039 WB ; Mouse.

PubMed:33511306

[IF=1.947] Gao M et al. Activating the interleukin-6-Gp130-STAT3 pathway ameliorates ventricular electrical stability in myocardial infarction rats by modulating neurotransmitters in the paraventricular nucleus. BMC Cardiovasc Disord. 2020 Feb 5;20(1):60. IHSLCP&WB ; Rat.

PubMed:6424466

[IF=2.25] Li H et al. MicroRNA-204-5p suppresses IL6-mediated inflammatory response and chemokine generation in HK-2 renal tubular epithelial cells by targeting IL6R. Biochem Cell Biol. 2019 Apr;97(2):109-117. WB ; Human.

PubMed:30110560

[IF=3.457] Ling L et al. MicroRNA-30e promotes hepatocyte proliferation and inhibits apoptosis in cecal ligation and puncture-induced sepsis through the JAK/STAT signaling pathway by binding to FOSL2.Biomed Pharmacother. 2018 Aug;104:411-419. WB ; Rat.

PubMed:29787988

[IF=2.559] Bi J et al. PPARγ alleviated hepatocyte steatosis through reducing SOCS3 by inhibiting JAK2/STAT3 pathway. Biochem Biophys Res Commun. 2018 Apr 15;498(4):1037-1044. ICC&WB ; Rat.

PubMed:29550470

[IF=0.9] Zhang, Yu, et al. "JAK-STAT signaling regulation of chicken embryonic stem cell differentiation into male germ cells." In Vitro Cellular & Developmental Biology-Animal (2017): 1-16. WB ; Chicken.

PubMed:28597334

[IF=3.23] Sur, Swastika, et al. "Increased Expression of Phosphorylated Polo-Like Kinase 1 and Histone in Bypass Vein Graft and Coronary Arteries following Angioplasty." PloS one 11.1 (2016): e0147937. IF(IHSLCP) ; Pig.

PubMed:26820885

[IF=3.23] Lee E-J, Lee SJ, Kim J-H, Kim K-J, Yang S-H, Jeong K-Y, et al. (2016) Radiation Inhibits Interleukin-12 Production via Inhibition of SLCRel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells. PLoS ONE 11(1): e0146463. WB ; Mouse.

PubMed:26745884