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Home > Product > Antibody > Rabbit Anti-LAMP1 antibody
CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane
Cat:
SL1970R
Species Reactivity：
Human,Mouse,Rat,Pig,
Immunogen：
KLH conjugated synthetic peptide derived from human LAMP-1:301-417/417
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Lane 1: Human HT-1080 cell lysates Lane 2: Human JAR cell lysates Primary: Anti-LAMP1 (SL1970R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDaObserved band size: 110 kDaParaformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP1) Polyclonal Antibody, Unconjugated (SL1970R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (LAMP1) Polyclonal Antibody, Unconjugated (SL1970R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-cy3) at 37°C for 90 minutes, DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.Blank control (blue line): U937 (blue)(fixed with 2% paraformaldehyde for 10 min at room temperature). Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (SL1970R);Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<;Dilution: 1μg /test. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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Datasheet:


Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Function:
Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Post-translational modifications:
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.

Similarity:
Belongs to the LAMP family.

SWISS:
P11279

Gene ID:
3916

Database links:

Entrez Gene: 3916 Human

Entrez Gene: 16783 Mouse

Entrez Gene: 25328 Rat

Omim: 153330 Human

SwissProt: P11279 Human

SwissProt: P11438 Mouse

SwissProt: P14562 Rat

Unigene: 494419 Human

Unigene: 16716 Mouse

Unigene: 475822 Mouse

Unigene: 40177 Rat



CD107a可很好的反映NK细胞活性，其表达水平的上调与NK分泌细胞因子的增多及NK对靶细胞的杀伤作用的增强高度相关，采用流式检测，CD107a还可鉴定出一群无细胞因子分泌的活性NK细胞。 Picture

Sample:
Lane 1: Human HT-1080 cell lysates
Lane 2: Human JAR cell lysates
Primary: Anti-LAMP1 (SL1970R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 110 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP1) Polyclonal Antibody, Unconjugated (SL1970R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (LAMP1) Polyclonal Antibody, Unconjugated (SL1970R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-cy3) at 37°C for 90 minutes, DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.
Blank control (blue line): U937 (blue)(fixed with 2% paraformaldehyde for 10 min at room temperature).
Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (SL1970R); Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<; Dilution: 1μg /test.
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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