Rabbit Anti-Heme Oxygenase 1 antibody

Home > Product > Antibody > Rabbit Anti-Heme Oxygenase 1 antibody

Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; HMOX1; HO 1; HO-1; HO; HO1; Hsp32; bK286B10; D8Wsu38e; HMOX1_HUMAN.

Cat:

SL2075R

Species Reactivity：

Human,Mouse,Rat,Pig,(predicted: Chicken,Dog,Cow,Horse,Rabbit,Sheep,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human HO-1:101-200/288

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti-Heme Oxygenase 1 (SL2075R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32 kDObserved band size: 33 kDSample: H9C2(Rat) Cell Lysate at 30 ugPrimary: Anti-Heme Oxygenase 1 (SL2075R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32 kDObserved band size: 32 kDSample: Kidney(Mouse) lysate at 30ug; Primary: Anti-Heme Oxygenase 1 (SL2075R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size :32 kDObserved band size : 32 kDSample: 293T Cell lysate at 30ug; Primary: Anti-Heme Oxygenase 1 (SL2075R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size :32 kDObserved band size : 32 kDSample: Lane 1: HepG2 (Human) Cell Lysate at 30 ugLane 2: A549 (Human) Cell Lysate at 30 ugLane 3: DU145 (Human) Cell Lysate at 30 ugLane 4: H9C2 (Human) Cell Lysate at 30 ugLane 5: 293T (Human) Cell Lysate at 30 ugPrimary: Anti-Heme Oxygenase 1 (SL2075R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32 kDObserved band size: 35 kDTissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Heme Oxygenase 1 Polyclonal Antibody, Unconjugated(SL2075R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control(blue): Mouse spleen (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Heme Oxygenase 1 antibody(SL2075R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The hemeoxygenase-1 calls that the hemoglobin oxidizes to synthesize the enzyme again-1( hemeoxygenase-1, HO-1) is the catalyst enzyme that a kind of hemoglobin declines the solution, under the NADPH and the cell dye P-450 revivification enzymes and the member oxygen functions, the catalyst HO-1 hemoglobin declines the solution as the courage green vegetable, CO and irons, the former revivification has the very strong anti- to oxidize the ability after become the red vegetable of courage , the latter is a kind of important letter to make the member.

Function:
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.

Subcellular Location:
Microsome. Endoplasmic reticulum.

Tissue Specificity:
Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

DISEASE:
Defects in HMOX1 are the cause of heme oxygenase 1 deficiency (HMOX1D) [MIM:614034]. A disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly.

Similarity:
Belongs to the heme oxygenase family.

SWISS:
P09601

Gene ID:
3162

Database links:

Entrez Gene: 442987 Dog

Entrez Gene: 3162 Human

Entrez Gene: 15368 Mouse

Entrez Gene: 24451 Rat

Omim: 141250 Human

SwissProt: Q5E9F2 Cow

SwissProt: P09601 Human

SwissProt: P14901 Mouse

Picture

Sample:
H9C2(Rat) Cell Lysate at 30 ug
Primary: Anti-Heme Oxygenase 1 (SL2075R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD

Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-Heme Oxygenase 1 (SL2075R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size :32 kD
Observed band size : 32 kD

Sample: 293T Cell lysate at 30ug;
Primary: Anti-Heme Oxygenase 1 (SL2075R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size :32 kD
Observed band size : 32 kD

Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: DU145 (Human) Cell Lysate at 30 ug
Lane 4: H9C2 (Human) Cell Lysate at 30 ug
Lane 5: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-Heme Oxygenase 1 (SL2075R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 35 kD

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Heme Oxygenase 1 Polyclonal Antibody, Unconjugated(SL2075R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control(blue): Mouse spleen (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Heme Oxygenase 1 antibody(SL2075R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Product Feedback Wall

Specific References (29) | SL2075R has been referenced in 29 publications.

[IF=3.117] Li J et al. Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway. Vet Res. 2019 Oct 21;50(1):83. WB ; Chicken.

PubMed:31639045

[IF=1.641] Feng Gao. et al. Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27 WB ; Bovine.

PubMed:33866621

[IF=3.263] Mahmoud E. Youssef. et al. α7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996 IHC ; Rat.

PubMed:33684450

[IF=4.545] Yingran Liang. et al. Crocin ameliorates arsenic trioxide‑induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713 WB ; Rat.

PubMed:32920515

[IF=3.04] Tawfik KM et al. Neuroprotective mechanisms of sildenafil and selenium in PTZ-kindling model: Implications in epilepsy.Eur J Pharmacol. 2018 Aug 15;833:131-144. IHSLCP ; Mouse.

PubMed:5967031

[IF=1.69] Xuan et al. Astaxanthin blocks preeclampsia progression by suppressing oxidative stress and inflammation. (2016) Mol.Med.Rep. 14:2697-704 IHSLCP ; Rat.

PubMed:27484589

[IF=1.49] Wang et al. Comparison of HMOX1 expression and enzyme activity in blue-shelled chickens and brown-shelled chickens. (2013) Genet.Mol.Biol. 36:282-6 WB ; Chicken.

PubMed:23885212

[IF=3.79] Bai, Juan, et al. "Central administration of tert-butylhydroquinone attenuates hypertension via regulating Nrf2 signaling in the hypothalamic paraventricular nucleus of hypertensive rats." Toxicology and Applied Pharmacology (2017). WB ; Rat.

PubMed:28842207

[IF=1.55] Wang, Yun, et al. "Fluoxetine protects against methamphetamine‑induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats." Molecular Medicine Reports 15.2 (2017): 673-136. WB ; Rat.

PubMed:28035393

[IF=3.71] Wei, Bo, et al. "Regulation of antioxidant system, lipids and fatty acid β-oxidation contributes to the cardioprotective effect of sodium tanshinone IIA sulphonate in isoproterenol-induced myocardial infarction in rats."Atherosclerosis (2013). WB ; Rat.

PubMed:23958267

[IF=2.81] Maïga, Souleymane, et al. "Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model." PLOS ONE 12.7 (2017): e0181067. WB ; Pig.

PubMed:28704481

[IF=6.244] Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282 WB ; Mouse.

PubMed:34307161

[IF=4.407] Caijun Zhao. et al. Kynurenic acid protects against mastitis in mice by ameliorating inflammatory responses and enhancing blood-milk barrier integrity. Mol Immunol. 2021 Sep;137:134 WB ; Mouse.

PubMed:34247099

[IF=6.992] Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;: WB ; Rat.

PubMed:10.1016/j.jare.2021.04.002

[IF=3.056] Xiao Wang. et al. Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis. Environ Sci Pollut R. 2021 Jan;28(1):936-946 WB ; Chicken.

PubMed:32827115

[IF=4.96] L.X. Li. et al. MiR-23a-5p exacerbates intestinal ischemia–reperfusion injury by promoting oxidative stress via targeting PPAR alpha. Biochem Pharmacol. 2020 Oct;36:114194 WB ; Rat.

PubMed:32800851

[IF=4.105] Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1α proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963 WB ; Human.

PubMed:33444648

[IF=5.076] Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev . 2020 Jul 9;2020:6428498. WB ; Human.

PubMed:32695257

[IF=7.338] Giraud S et al. The inhibition of eIF5A hypusination by GC7, a preconditioning protocol to prevent brain death‐induced renal injuries in a preclinical porcine kidney transplantation model. Am J Transplant. 2020 Dec;20(12):3326-3340. WB ; Pig.

PubMed:3480964

[IF=4.527] Han C et al. Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Ecotoxicol Environ Saf. 2020 Mar 15;191:110239. WB ; Chicken.

PubMed:31991393

[IF=1.581] Ni et al. Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling. (2015) Int.J.Clin.Exp.Pathol. 8:7752-61 WB ; Rat.

PubMed:26339340

[IF=1.58] Zhang et al. Protective effect of heme oxygenase-1 on Wistar rats with heart failure through the inhibition of inflammation and amelioration of intestinal microcirculation. (2015) J.Geriatr.Cardiol. 12:353-65 WB ; Rat.

PubMed:26346675

[IF=4.24] Wei et al. The cardioprotective effect of sodium tanshinone IIA sulfonate and the optimizing of therapeutic time window in myocardial ischemia/reperfusion injury in rats. (2014) Atherosclerosis. 235:318-27 WB ; Rat.

PubMed:24911635

[IF=0.27] Tao, Kai, Jinghai Chen, and Lingyun Wang. "Effects of berberine on the expressions of NRF2 and HO-1 in endothelial cells of diabetic rat." Biomedical Research 28.9 (2017): 3860-3864. WB, IHSLCP ; Rat.

PubMed:BiomedicalResearch(0970-938X)

[IF=1.07] Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597. WB ; Human.

PubMed:ISSN:1940-5901/IJCEM0037069

[IF=4.49] Wang, Yun, et al. "TSHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity." Oxidative Medicine and Cellular Longevity 2017 (2017). WB ; Rat.

PubMed:28303170

[IF=0.87] Zeng, X-P., et al. "Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity." Transplantation Proceedings. Vol. 49. , 2017. WB ; Rat.

PubMed:28219600

[IF=4.64] Song, Wei, et al. "Astroglial Heme Oxygenase-1 and the Origin of Corpora Amylacea in Aging and Degenerating Neural Tissues." Experimental Neurology (2014). IHSLCP ; Mouse.

PubMed:2488642

[IF=5.27] Lee, Bao-Hong, et al. "Dimerumic acid attenuates receptor for advanced glycation endproduct (RAGE) signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promoted methylglyoxal metabolism into D-lactate acid" Free Radical Biology and Medicine WB ; Human.

PubMed:23434766