Rabbit Anti-Adipose Triglyceride Lipase antibody

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PEDF R; Adipose Triglyceride Lipase; TTS 2.2; PEDFR; PEDF-R; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Desnutrin; EC 3.1.1.3; IPLA2 zeta; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA;

Cat:

SL3831R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human ATGL:401-504/504

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-F=1:100-500Flow-Cyt=3ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Ovary (Mouse) Lysate at 40 ugPrimary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Testis (Mouse) Lysate at 40 ugPrimary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Lung (Mouse) Lysate at 40 ugPrimary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 55 kDObserved band size: 55 kDBlank control: A431. Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (SL3831R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647Dilution: 3μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

Subunit:
Interacts with ASHD5; this association stimulates PNPLA2 triglyceride hydrolase activity.

Subcellular Location:
Lipid droplet. Cell membrane.

Tissue Specificity:
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).

Similarity:
Contains 1 patatin domain.

SWISS:
Q96AD5

Gene ID:
57104

Database links:

Entrez Gene: 57104 Human

Entrez Gene: 66853 Mouse

Entrez Gene: 361676 Rat

Omim: 609059 Human

SwissProt: Q96AD5 Human

SwissProt: Q8BJ56 Mouse

SwissProt: P0C548 Rat

Unigene: 654697 Human

Unigene: 29998 Mouse

Unigene: 19196 Rat

Pathway: Glycerolipid metabolism; triacylglycerol degradation. Picture

Sample: Ovary (Mouse) Lysate at 40 ug
Primary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample: Lung (Mouse) Lysate at 40 ug
Primary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- Adipose Triglyceride Lipase (SL3831R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (SL3831R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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