Rabbit Anti-TUBA1C antibody

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Alpha tubulin 6; Alpha tubulin isotype M alpha 6; bcm948; M[a]6; TUBA1C; Tubulin alpha 1C chain; Tubulin alpha-1C chain; tubulin alpha 6; Tubulin alpha 6 chain; tubulin, alpha 1c; TBA1C_HUMAN; Alpha-tubulin 6; Tubulin alpha-6 chain.

Cat:

SL5118R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Tubulin alpha 6:51-150/449

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebral cortex (Mouse) Lysate at 40 ugPrimary: Anti- TUBA1C (SL5118R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 52 kDSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-TUBA1C (SL5118R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 49 kDSample:A549 Cell (Human) Lysate at 30 ugPrimary: Anti- TUBA1C (SL5118R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49kDObserved band size: 49kDSample: K562(Human) Cell Lysate at 40 ugHela(Human) Cell Lysate at 40 ugPrimary: Anti-TUBA1C (SL5118R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 49 kDParaformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBA1C) Polyclonal Antibody, Unconjugated (SL5118R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- TUBA1C antibody(SL5118R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit:
Dimer of alpha and beta chains.

Subcellular Location:
Cytoplasm, cytoskeleton.

Post-translational modifications:
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a SLCterminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the SLCterminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Similarity:
Belongs to the tubulin family.

SWISS:
Q9BQE3

Gene ID:
84790

Database links:

Entrez Gene: 84790 Human

Entrez Gene: 504244 Cow

Entrez Gene: 22146 Mouse

Entrez Gene: 300218 Rat

SwissProt: Q3ZCJ7 Cow

SwissProt: Q9BQE3 Human

SwissProt: P68373 Mouse

SwissProt: Q6AYZ1 Rat

Unigene: 725987 Human

Unigene: 88212 Mouse

Unigene: 154526 Rat

Picture

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-TUBA1C (SL5118R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD

Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti- TUBA1C (SL5118R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49kD
Observed band size: 49kD

Sample:
K562(Human) Cell Lysate at 40 ug
Hela(Human) Cell Lysate at 40 ug
Primary: Anti-TUBA1C (SL5118R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBA1C) Polyclonal Antibody, Unconjugated (SL5118R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti- TUBA1C antibody(SL5118R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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