Rabbit Anti-NARG2 antibody | Sunlong Medical

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BRCC1; NARG 2; NARG2_HUMAN; NMDA receptor regulated protein 2; NMDA receptor-regulated protein 2.

Cat:

SL5968R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human NARG2:201-300/982

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Rat) Lysate at 40 ugRaw264.7 (Mouse) Cell Lysate at 30 ugPrimary: Anti- NARG2 (SL5968R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 110 kDObserved band size: 110 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARG2) Polyclonal Antibody, Unconjugated (SL5968R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: U-2OS(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).Blank control: RSC96(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).Blank control: HCCLM3(blue)Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).Blank control: U-2OS(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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NARG2 is expressed at relatively high levels in dividing and immature cells, and is down-regulated upon terminal differentiation. NARG2 is a novel (S/T)PXX motif-containing nuclear protein; this motif is present in many transcription factors as well as other regulatory proteins that bind to DNA such as histones and RNA polymerase II. Three different isoforms exist.

Subcellular Location:
Nucleus (By similarity).

Tissue Specificity:
Expressed at low levels in lung and testis.

SWISS:
Q659A1

Gene ID:
79664

Database links:

Entrez Gene: 79664 Human

Entrez Gene: 93697 Mouse

Omim: 610835 Human

SwissProt: Q659A1 Human

SwissProt: Q3UZ18 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARG2) Polyclonal Antibody, Unconjugated (SL5968R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control: U-2OS(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

Blank control: HCCLM3(blue)
Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).

Blank control: U-2OS(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

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