Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum.
Function:
E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Similarity:
Contains 1 RING-CH-type zinc finger.
SWISS:
Q9H992
Gene ID:
64844
Database links:
Entrez Gene: 64844 Human
Entrez Gene: 57438 Mouse
Entrez Gene: 311059 Rat
Omim: 613334 Human
SwissProt: Q9H992 Human
SwissProt: Q9WV66 Mouse
SwissProt: Q5R9W2 Orangutan
SwissProt: Q5XI50 Rat
Unigene: 529272 Human
Unigene: 260635 Mouse
Unigene: 117620 Rat
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Sample:
Siha(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti-MARCH7 (SL9341R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 73 kD
Sample:
Siha(Human) Cell Lysate at 30 ug
Primary: Anti-MARCH7 (SL9341R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 73 kD
Sample:
Siha(human)cell Lysate at 30 ug
MCF-7(human)cell Lysate at 30 ug
U251(human)cell Lysate at 30 ug
testis(rat) Lysate at 40 ug
Primary: Anti-MARCH7 (SL9341R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78kD
Observed band size: 73 kD
Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-MARCH7 (SL9341R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78/74 kD
Observed band size: 78/74 kD
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (SL9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (SL9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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