Rabbit Anti-Arginase II antibody

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ARG 2; ARG2; ARGI2_HUMAN; Arginase-2; Arginase 2Arginase2; Arginase liver; Arginase type II; Arginase II; ArginaseII; Arginase-II; Arginase1; Arginase-2, mitochondrial; Kidney-type arginase; Non-hepatic arginase; Type II arginase; .

Cat:

SL11397R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Arginase II:181-290/354

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:DU145(human)cell Lysate at 30 ugPrimary: Anti-Arginase II (SL11397R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36kDObserved band size: 36 kDSample: Small intestine (Mouse) Lysate at 40 ugPrimary: Anti-Arginase II (SL11397R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample: Small intestine (Mouse) Lysate at 40 ugPrimary: Anti-Arginase II (Bs- 11397R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated (SL11397R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated (SL11397R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human MCF-7 cells;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Arginase II Polyclonal Antibody, Unconjugated(SL11397R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea (1). The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster (2-4). Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location (5,6). Specifically, arginase II localizes to the mitochondria (5,6). Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver (5,6). The human arginase II gene, which maps to chromosome 14q24.1, encodes a 354 amino acid protein (3,5-7). In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence (5,6).

Function:
May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.

Subunit:
Homotrimer.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas.

Similarity:
Belongs to the arginase family.

SWISS:
P78540

Gene ID:
384

Database links:

Entrez Gene: 384 Human

Entrez Gene: 11847 Mouse

Entrez Gene: 29215 Rat

Omim: 107830 Human

SwissProt: P78540 Human

SwissProt: O08691 Mouse

SwissProt: O08701 Rat

Unigene: 226007 Human

Unigene: 723133 Human

Unigene: 3506 Mouse

Unigene: 11055 Rat

Picture

Sample: Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-Arginase II (SL11397R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Sample:
Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-Arginase II (Bs- 11397R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated (SL11397R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated (SL11397R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human MCF-7 cells;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Arginase II Polyclonal Antibody, Unconjugated(SL11397R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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