Rabbit Anti-phospho-ZCWCC1 (Ser739) antibody

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AC004542.C22.1.; p-MORC2(Ser739); phospho-ZCWCC1(Ser739); CW type with coiled coil domain 1; KIAA0852; ZCW3; ZCWCC1; Zinc finger; Zinc finger CW type coiled coil domain protein 1; Zinc finger CW type with coiled coil domain 1; Zing finger CW type 3 zinc f

Cat:

SL10016R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human MORC2 around the phosphorylation site of Ser739:KR(p-S)VA

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7(Human) Cell Lysate at 30 ugK562(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114 kDObserved band size: 114 kDSample: U2os(Human) Cell Lysate at 30 ugPrimary: Anti-phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114 kDObserved band size: 114 kDSample: 293T(Human) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114 kDObserved band size: 114 kDSample: MCF-7(Human) Cell Lysate at 30 ugK562(Human) Cell Lysate at 30 ug293T(Human) Cell Lysate at 30 ugHepG2(Mouse) Cell Lysate at 30 ugPrimary: Anti-phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114 kDObserved band size: 114 kDSample: Testis (Mouse) Lysate at 40 ugPrimary: Anti- phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114 kDObserved band size: 114 kDParaformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ZCWCC1 (Ser739)) Polyclonal Antibody, Unconjugated (SL10016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-ZCWCC1(Ser739) Polyclonal Antibody, Unconjugated(SL10016R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The CW domain is a structural module found in many vertebrate, parasitic and plant proteins. It consists of a mononuclear four-cysteine zinc-finger domain that plays a role in DNA binding, chromatin methylation and early embryonic development. ZCWCC1 (zinc finger CW-type coiled-coil domain protein 1), also known as MORC2 (MORC family CW-type zinc finger protein 2) or ZCW3, is a 1,032 amino acid protein that contains one CW-type zinc finger domain. ZCWCC1 is located on chromosome 22 and is ubiquitously expressed with highest expression in pancreas, smooth muscle and testis. Expression of ZCWCC1 is upregulated in hypoxia, a pathological condition characterized by an inadequate supply of oxygen in the blood.

Function:
May act as a transcriptional repressor. Down-regulates CA9 expression.

Subunit:
Interacts with HDAC4.

Subcellular Location:
Nucleus. Cytoplasm, cytosol. Note=Mainly located in the nucleus.

Tissue Specificity:
Highly expressed in smooth muscle, pancreas and testis.

Similarity:
Contains 1 CW-type zinc finger.

SWISS:
Q9Y6X9

Gene ID:
22176

Database links:

Entrez Gene: 22176 Human

Entrez Gene: 74522 Mouse

Entrez Gene: 289736 Rat

SwissProt: Q9Y6X9 Human

Unigene: 143168 Human

Unigene: 555918 Human

Unigene: 139127 Mouse

Picture

Sample:
U2os(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD

Sample:
293T(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD

Sample:
MCF-7(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
HepG2(Mouse) Cell Lysate at 30 ug
Primary: Anti-phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- phospho-ZCWCC1 (Ser739) (SL10016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ZCWCC1 (Ser739)) Polyclonal Antibody, Unconjugated (SL10016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ZCWCC1(Ser739) Polyclonal Antibody, Unconjugated(SL10016R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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