Rabbit Anti-ADGRE1 antibody

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F4/80; Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1;

Cat:

SL7058R

Species Reactivity：

Human,Mouse,(predicted: Rat,Pig,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human ADGRE1:701-800/886<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=2μg/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: U87MG(Human) Cell Lysate at 30 ugPrimary: Anti- EMR1 (SL7058R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 95 kDObserved band size: 95 kDSample: A549(Human) Cell Lysate at 30 ugPrimary: Anti- EMR1 (SL7058R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 95 kDObserved band size: 95 kDSample: A431(Human) Cell Lysate at 30 ugPrimary: Anti-EMR1 (SL7058R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 95 kDObserved band size: 95 kDBlank control:A431. Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (SL7058R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488Dilution: 0.5ug/Test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-EMR1 antibody (SL7058R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: Mouse brain. Primary Antibody (green line): Rabbit Anti-EMR1 antibody (SL7058R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Positive control: mouse Splenocytes(2% Paraformaldehyde-fixed )Isotype Control Antibody: Rabbit IgG Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA; Primary Antibody : rabbit Anti-EMR1 SL7058R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its SLCterminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Function:
Could be involved in cell-cell interactions.

Subunit:
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains. Contains 1 GPS domain.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Wide expression; increased levels in peripheral blood mononuclear cells.

Similarity:
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains.
Contains 1 GPS domain.

SWISS:
Q14246

Gene ID:
2015

Database links:

Entrez Gene: 2015 Human

Entrez Gene: 13733 Mouse

Omim: 600493 Human

SwissProt: Q14246 Human

SwissProt: Q61549 Mouse

Unigene: 2375 Human

Unigene: 2254 Mouse

Picture

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- EMR1 (SL7058R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-EMR1 (SL7058R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD

Blank control:A431.
Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (SL7058R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-EMR1 antibody (SL7058R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: Mouse brain.
Primary Antibody (green line): Rabbit Anti-EMR1 antibody (SL7058R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Positive control: mouse Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA; Primary Antibody : rabbit Anti-EMR1 SL7058R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA.

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