Rabbit Anti-P2Y9 antibody | Sunlong Medical

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5730485F04Rik; G protein coupled receptor 23; G-protein coupled receptor 23; GPR23; LPA receptor 4; LPA-4; LPA4; Lpar4; LPAR4_HUMAN; Lysophosphatidic acid receptor 4; Lysophosphatidic Acid Receptor P2Y9; P2RY9; P2Y purinoceptor 9; P2Y5 like; P2Y5-like rec

Cat:

SL12074R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human P2Y9:175-270/370<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugOvary (Mouse) Lysate at 40 ugPrimary: Anti-P2Y9 (SL12074R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 57 kDSample: HL-60(Human) Cell Lysate at 30 ugK562(Human) Cell Lysate at 30 ugPrimary: Anti-P2Y9 (SL12074R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 57 kDSample: Brain (mouse) Lysate at 40 ugPrimary: Anti- P2Y9 (SL12074R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 48 kDSample: k562 (human)cell Lysate at 40 ugPrimary: Anti- P2Y9 (SL12074R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 48 kDSample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Ovary tissue lysates Lane 3: Human HL60 cell lysates Lane 4: Human U87MG cell lysates Primary: Anti-P2Y9 (SL12074R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDaObserved band size: 50 kDa Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (SL12074R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (SL12074R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (SL12074R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Nucleotides are emerging as important extracellular signaling molecules that mediate several effects, such as proliferation, differentiation , chemotaxis and cytokine release. The P2 receptor family is activated by the binding of nucleotides and is divided into two subfamilies, P2X and P2Y. The P2X receptor family is comprised of ligand-gated ion channels that allow for the increased permeability of calcium into the cell in response to extracellular ATP. The P2Y receptor family are G protein-coupled receptors which mediate the effects of extracellular nucleotides, primarily through the activation of phospholipase C. To some extent, the P2Y receptors can also activate potassium channels or, alternatively, inhibit adenylate cyclase and N-type calcium channels in response to extracellular nucleotides. P2Y9 is activated by lysophosphatidic acid (LPA), a lipid mediator involved in cell proliferation, differentiation, survival and death. In hamsters, P2Y9 mRNA is significantly expressed in ovary tissue compared to other tissues, and innervation with 1-oleoly LPA increases intracellular calcium ion concentration and stimulates adenylyl cyclase activity. P2Y9 is structurally related to nucleotide receptors, and shares 20-24% amino acid homology with the three other LPA receptors (LPA1, LPA2, LPA3).

Function:
Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
Q8BLG2

Gene ID:
78134

Database links:
UniProtKB/Swiss-Prot: Q99677.1

Picture

Sample:
HL-60(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-P2Y9 (SL12074R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 57 kD

Sample: Brain (mouse) Lysate at 40 ug
Primary: Anti- P2Y9 (SL12074R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 48 kD

Sample: k562 (human)cell Lysate at 40 ug
Primary: Anti- P2Y9 (SL12074R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 48 kD

Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Ovary tissue lysates
Lane 3: Human HL60 cell lysates
Lane 4: Human U87MG cell lysates
Primary: Anti-P2Y9 (SL12074R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 50 kDa

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (SL12074R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (SL12074R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (SL12074R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

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