Rabbit Anti-NR1H4 antibody | Sunlong Medical

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Bile Acid Receptor NR1H4; BAR; FXR; Farnesoid X activated receptor; Farnesoid X receptor; Farnesoid X-activated receptor; Farnesol receptor HRR 1; Farnesol receptor HRR-1; Farnesol receptor HRR1; FXR; HRR 1; HRR1;NR1H4_HUMAN; Nuclear receptor subfamily 1

Cat:

SL12867R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human FXR/Bile Acid Receptor NR1H4:175-280/486

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3ug/testICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2 (Human) CellLysate at 30 ugPrimary: Anti-NR1H4 (SL12867R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDSample: Large intestine (Mouse) Lysate at 40 ugPrimary: Anti-NR1H4 (SL12867R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDSample: HepG2 Cell (Human) Lysate at 40 ugPrimary: Anti-NR1H4 (SL12867R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDSample: Liver (Mouse) Lysate at 40 ugPrimary: Anti- NR1H4 (SL12867R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDSample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-NR1H4 (SL12867R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDBlank control:A549. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (SL12867R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 3μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and binds DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, and several novel receptors including LXR (also referred to as RLD-1) and FXR. FXR and LXR fall into a category of proteins termed “orphan receptors” because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid.

Function:
Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.

Subunit:
eterodimer of NR1H4 and RXR. After activation by agonist binding, interacts with a coactivator, NCOA1 or NCOA2 (By similarity). Interacts with CARM1 and SMARD1.

Subcellular Location:
Nucleus.

Post-translational modifications:
Methylation may increase transactivation of target genes.

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
Q96RI1

Gene ID:
9971

Database links:

Entrez Gene: 9971 Human

Entrez Gene: 20186 Mouse

Entrez Gene: 60351 Rat

Omim: 603826 Human

SwissProt: Q96RI1 Human

SwissProt: Q3V1T8 Mouse

SwissProt: Q60641 Mouse

SwissProt: Q5XI75 Rat

SwissProt: Q62735 Rat

Unigene: 282735 Human

Unigene: 3095 Mouse

Unigene: 42943 Rat

Picture

Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-NR1H4 (SL12867R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-NR1H4 (SL12867R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- NR1H4 (SL12867R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-NR1H4 (SL12867R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Blank control:A549.
Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (SL12867R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (14) | SL12867R has been referenced in 14 publications.

[IF=2.629] Chen Lisheng. et al. Paeoniflorin Protects against ANIT-Induced Cholestatic Liver Injury in Rats via the Activation of SIRT1-FXR Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8479868 WB ; rat.

PubMed:34512782

[IF=4.679] Mengzhi Zou. et al. The role of invariant natural killer T cells and associated immunoregulatory factors in triptolide-induced cholestatic liver injury. Food Chem Toxicol. 2020 Dec;146:111777 WB ; Mouse.

PubMed:32987112

[IF=4.545] Yong-li Hua. et al. Baitouweng Tang ameliorates DSS-induced ulcerative colitis through the regulation of the gut microbiota and bile acids via pathways involving FXR and TGR5. Biomed Pharmacother. 2021 May;137:11164 WB ; Mouse.

PubMed:33578232

[IF=4.18] Li T et al. Picroside II protects against cholestatic liver injury possibly through activation of farnesoid X receptor. Phytomedicine,2019. 153153. WB ; Mouse.

PubMed:doi:10.1016/j.phymed.2019.153153

[IF=4.414] Liu Y et al. Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD. J Nutr Biochem. 2019 Apr 4;69:53-62. WB ; Mouse.

PubMed:31055233

[IF=3.547] Yuan Z et al. A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation. Toxicology. 2019 Feb 15;414:45-56. WB ; Mouse.

PubMed:30633930

[IF=3.71] Zhou et al. Retinoic acid induces macrophage cholesterol efflux and inhibits atherosclerotic plaque formation in apoE-deficient mice. (2015) Br.J.Nutr. 114:509-18 WB ; Mouse.

PubMed:26201974

[IF=3.77] Yang, Tingting, et al. "Early indications of ANIT-induced cholestatic liver injury: Alteration of hepatocyte polarization and bile acid homeostasis." Food and Chemical Toxicology (2017). IHSLCP ; Rat.

PubMed:28986171

[IF=3.85] Li, Xiaojiaoyang, et al. "UDCA and CDCA alleviate 17α-ethinylestradiol-induced cholestasis through PKA-AMPK pathways in rats." Toxicology and Applied Pharmacology 311 (2016): 12-25. WB ; Human.

PubMed:27743861

[IF=3.52] Yu, Linxi, et al. "Protective effects of SRT1720 via the HNF1α/FXR signalling pathway and anti-inflammatory mechanisms in mice with estrogen-induced cholestatic liver injury." Toxicology Letters (2016). WB ; Mouse.

PubMed:27818225

[IF=3.85] Guo, Hong-li, et al. "Pyrazinamide induced rat cholestatic liver injury through inhibition of FXR regulatory effect on bile acid synthesis and transport."Toxicological Sciences (2016): kfw098. WB ; Rat.

PubMed:27255380

[IF=4.4] Yu et al. SRT1720 Alleviates ANIT-Induced Cholestasis in a Mouse Model. (2017) Front.Pharmaco. 8:256 WB ; Mouse.

PubMed:28553227

[IF=3.616] Zhao G et al. Adaptive homeostasis of the vitamin D–vitamin D nuclear receptor axis in 8-methoxypsoralen-induced hepatotoxicity. (2018) Toxicology and Applied Pharmacology. Jan 1;362:150-158. WB ; Rat.

PubMed:30419252

[IF=8.4] Zhang, Qiankun, et al. "Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study." Biomaterials (2017) WB ; Mouse.

PubMed:29017079