This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants.
Function:
Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHSLV8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Subcellular Location:
Secreted and Cell membrane.
Tissue Specificity:
Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
Post-translational modifications:
The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
Similarity:
Belongs to the CD200R family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.
SWISS:
Q6Q8B3
Gene ID:
131450
Database links:
Entrez Gene: 131450 Human
Omim: 607546 Human
SwissProt: Q8TD46 Human
Unigene: 309158 Human
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Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (SL7351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 53 kD
Sample:hela(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (SL7351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 58 kD
Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (SL7351R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (SL7351R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CD200R) Polyclonal Antibody, Unconjugated (SL7351R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Blank control:Hepg2 Cells(blue).
Primary Antibody: Rabbit Anti-CD200R/AF647 Conjugated antibody (SL7351R-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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