This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Function:
p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).
Subunit:
Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and
Subcellular Location:
Cytoplasm, cytoskeleton. Cell projection.
Similarity:
Belongs to the ARPC3 family.
SWISS:
O15145
Gene ID:
10094
Database links:
Entrez Gene: 10094 Human
Entrez Gene: 56378 Mouse
Entrez Gene: 288669 Rat
Entrez Gene: 415204 Zebrafish
Omim: 604225 Human
SwissProt: O15145 Human
SwissProt: Q9JM76 Mouse
Unigene: 524741 Human
Unigene: 275942 Mouse
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Protein: intestinal(mouse) lysate at 30ug;
Primary: rabbit Anti-p21-ARC (SL17589R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size:20 kD
Observed band size: 20 kD
Protein:
intestinal(mouse) lysate at 30ug;
Raji(human) lysate at 30ug;
Primary: rabbit Anti-p21-ARC (SL17589R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size:20 kD
Observed band size: 20 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- p21-ARC (SL17589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-p21-ARC (SL17589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p21-ARC) Polyclonal Antibody, Unconjugated (SL17589R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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