[email protected]
Home > Product > Antibody > Rabbit Anti-Ki-67 antibody
Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_MOUSE.
Cat:
SL23103R
Species Reactivity：
Human,Mouse,
Immunogen：
KLH conjugated synthetic peptide derived from mouse Ki-67:1231-1330/2938
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample:MCF-7 Cell (human) Lysate at 40 ugPrimary: Anti-Ki67(SL23103R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 358kDObserved band size: 358kDSample: NIH/3T3(Mouse) Cell Lysate at 30 ugPrimary: Anti-Ki-67 (SL23103R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 358 kDObserved band size: 358 kD Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (SL23103R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (SL23103R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (SL23103R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control (blue line): mouse blood (fixed with 80% methanol (5 min at -20℃). Cells stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (SL23103R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
More
prev next
Unit:
Price: $
Product PDFs
Datasheet:


Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Function:
Thought to be required for maintaining cell proliferation.

Subcellular Location:
Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Similarity:
Contains 1 FHA domain.

SWISS:
Q91VE6

Gene ID:
17345

Database links:

Entrez Gene: 4288 Human

Entrez Gene: 17345 Mouse

Entrez Gene: 29242 Rat

Omim: 176741 Human

SwissProt: P9213 Human

SwissProt: Q91VE6 Mouse

SwissProt: Q5RJM0 Rat

Unigene: 689823 Human

Unigene: 80976 Human

Unigene: 4078 Mouse

Unigene: 233802 Rat



细胞增殖标志物(Proliferation Marker)
Ki67与PCNA一样，为细胞增殖的一种标记，在细胞凋亡中S、G2 、M期均有表达，G0期缺如。 Ki-67增殖指数高低与许多肿瘤的分化程度、浸润、转移以及预后密切相关，因此被广泛作为各种恶性肿瘤的必检项目之一。 Picture

Sample:MCF-7 Cell (human) Lysate at 40 ug
Primary: Anti-Ki67(SL23103R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 358kD
Observed band size: 358kD
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Ki-67 (SL23103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 358 kD
Observed band size: 358 kD
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (SL23103R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (SL23103R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (SL23103R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (blue line): mouse blood (fixed with 80% methanol (5 min at -20℃). Cells stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (SL23103R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1μg /test.
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.