Rabbit Anti-HOXc8 antibody | Sunlong Medical

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Hox-3.1; Homeo box 3A; Homeo box 8; Homeobox 3A; Homeobox C8; Homeobox protein Hox C8; Homeobox protein Hox-C8; Homeobox protein HoxC8; Homeobox protein R4; Homolog of mouse Hox 3.1; HOX 3; Hox 3.1 mouse homolog of; HOX 3A; HOX C8; Hox-3.1, MOUSE, HOMOLOG

Cat:

SL0394R

Species Reactivity：

Human,Rat,(predicted: Mouse,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human HOXc8:51-130/242

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain(Rat) lysate at 30ug; Liver(Rat) lysate at 30ug; Primary: Anti-HOXc8 (SL0394R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; Predicted band size : 27kDObserved band size : 27kD, 43kDSample: Liver(Rat) Lysate at 40 ugPrimary: Anti-HOXc8 (SL0394R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 27 kDParaformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (SL0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (SL0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (SL0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene may play a role in the regulation of cartilage differentiation. It could also be involved in chondrodysplasias or other cartilage disorders.

Function:
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Subunit:
Interacts with SMAD1 and HOMEZ.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
P31273

Gene ID:
3224

Database links:

Entrez Gene: 607052 Dog

Entrez Gene: 3224 Human

Entrez Gene: 15426 Mouse

Omim: 142970 Human

SwissProt: P31273 Human

SwissProt: P09025 Mouse

Unigene: 664500 Human

Unigene: 6167 Mouse

HOXc8基因是HOX基因家族中的一员，研究小鼠中HOXc8蛋白的位置、结构以及HOXc8蛋白在小鼠胚胎发育中的表达与调控、小鼠神经系统的发育均已被广泛重视.
HOXc8蛋白的表达对APP基因的表达有抑制作用,有学者认为HOXc8基因可能与Alzheimer's病的发病有关.
目前对哺乳动物同源盒基因在中枢神经系统发育中的广泛表达以及在胚胎发育中的区域特异性表达方式被认为是非常重要的，已引起科学家们的广泛重视。 Picture

Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-HOXc8 (SL0394R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (SL0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (SL0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (SL0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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