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Home > Product > Antibody > Rabbit Anti-LDL receptor antibody
Low-density lipoprotein receptor precursor; LDL-R; LDL R; FH; FHC; LDL R; LDLR; Low Density Lipoprotein Receptor; Low density lipoprotein receptor familial hypercholesterolemia; LDLR_HUMAN.
Cat:
SL0705R
Species Reactivity：
Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,Guinea Pig,)
Immunogen：
KLH conjugated synthetic peptide derived from human LDL-R:781-860/860<Cytoplasmic>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1μg/TestICC=1:100not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: K562(Human) Cell Lysate at 30 ugRaw264.7(Mouse) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugSW96(Human) Cell Lysate at 30 ugPrimary: Anti-LDL receptor (SL0705R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 92 kDObserved band size: 120 kDSample:Human colon Lysate at 45ug; Primary: Anti-LDL receptor (SL0705R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 92kDObserved band size : 92kDHepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (SL0705R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control：A431. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL0705R),Dilution: 1μg /10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody:Goat anti-rabbit IgG-AF647,Dilution: 1μg /test. ProtocolA431 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary Antibody (green line): Rabbit Anti-LDLreceptor antibody (SL0705R) ;Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL0705R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Product PDFs
Datasheet:


The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.

Function:
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HISLV1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

Subunit:
Interacts with LDLRAP1. Interacts with SNX17. Interacts with HCV E1/E2 heterodimer. Interacts with HISLV1 Tat.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein. Note=Found distributed from the plasma membrane to intracellular compartments.

Tissue Specificity:
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HISLV1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

Post-translational modifications:
N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.

Similarity:
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats.

SWISS:
P01130

Gene ID:
3949

Database links:

Entrez Gene: 3949 Human

Entrez Gene: 16835 Mouse

Entrez Gene: 300438 Rat

Omim: 606945 Human

SwissProt: P01130 Human

SwissProt: P35951 Mouse

SwissProt: P35952 Rat

Unigene: 213289 Human

Unigene: 728190 Human

Unigene: 3213 Mouse

Unigene: 10483 Rat



低密度脂蛋白受体 LDLR是一种存在于细胞表面的、可识别多种配体的脂蛋白受体，在体内对于富含甘油三酯的脂蛋白代谢非常重要;
LDL R目前主要用于代谢及肿瘤方面的研究。 Picture

Sample:
K562(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
SW96(Human) Cell Lysate at 30 ug
Primary: Anti-LDL receptor (SL0705R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 120 kD
Sample:Human colon Lysate at 45ug; Primary: Anti-LDL receptor (SL0705R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 92kD Observed band size : 92kD
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (SL0705R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control：A431.
Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL0705R),Dilution: 1μg /10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody:Goat anti-rabbit IgG-AF647,Dilution: 1μg /test.
Protocol
A431 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (green line): Rabbit Anti-LDLreceptor antibody (SL0705R) ; Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL0705R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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