Rabbit Anti-IL18 antibody | Sunlong Medical

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Interleukin-18; interferon gamma-inducing factor IGIF; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; Iboctadekin; IFN gamma inducing factor; IGIF; IL 1 gamma; IL 18; IL-18; IL 1g; IL1 gamma; IL18 protein; IL1F4; IL1g; I

Cat:

SL0529R

Species Reactivity：

Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from mouse IL-18:51-120/192

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Eye (Mouse) Lysate at 40 ugPrimary: Anti-IL-18 (SL0529R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 17 kDObserved band size: 18 kDParaformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-18) Polyclonal Antibody, Unconjugated (SL0529R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-18) Polyclonal Antibody, Unconjugated (SL0529R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(SL0529R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(SL0529R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions.

Function:
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Subcellular Location:
Secreted.

Similarity:
Belongs to the IL-1 family.

SWISS:
P70380

Gene ID:
16173

Database links:

Entrez Gene: 726 Human

Entrez Gene: 16173 Mouse

Omim: 600953 Human

SwissProt: Q14116 Human

SwissProt: P70380 Mouse

Unigene: 83077 Human

Unigene: 1410 Mouse

IL-18是巨噬细胞分泌的一个重要因子，它具有多种免疫功能，能诱导产生ＩＦＮγ，无论在病毒感染早期或特异性细胞免疫应答效应阶段都会出现，因此IL-18主要参与免疫应答及炎性细胞浸润的改变。Interleukin-18 又称inteferon-gamma inducing factor(IGIF)，在辅助T细胞的应答反应中起重要作用。IL-18和IL-1一起，既参与先天免疫，又是后天免疫的构成因素。 Picture

Sample:Eye (Mouse) Lysate at 40 ug
Primary: Anti-IL-18 (SL0529R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 18 kD

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-18) Polyclonal Antibody, Unconjugated (SL0529R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-18) Polyclonal Antibody, Unconjugated (SL0529R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(SL0529R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(SL0529R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall

Specific References (21) | SL0529R has been referenced in 21 publications.

[IF=2.479] Wangyu Wu. et al. Cholecalciferol pretreatment ameliorates ischemia/reperfusion-induced acute kidney injury through inhibiting ROS production, NF-κB pathway and pyroptosis. Acta Histochem. 2022 May;124:151875 IF ; Mouse.

PubMed:35334282

[IF=9.811] Nicholas Andrikopoulos. et al. Inhibition of Amyloid Aggregation and Toxicity with Janus Iron Oxide Nanoparticles. Chem Mater. 2021;XXXX(XXX):XXX-XXX WB ; Mouse.

PubMed:10.1021/acs.chemmater.1c01947

[IF=16.907] Yunxiang Sun. et al. Spontaneous formation of β-sheet nano-barrels during the early aggregation of Alzheimer’s amyloid beta. Nano Today. 2021 Jun;38:101125 WB ; Mouse.

PubMed:10.1016/j.nantod.2021.101125

[IF=3.869] Kong Dejun. et al. IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury. Stem Cells Int. 2020;2020:8883636 WB ; Rat.

PubMed:32849879

[IF=2.615] Wei Yu. et al. Curcumin suppresses doxorubicin-induced cardiomyocyte pyroptosis via a PI3K/Akt/mTOR-dependent manner. Cardiovasc Diagn The. 2020 Aug; 10(4): 752–769 WB ; Mouse.

PubMed:32968631

[IF=3.105] Xiao-ling Zhang. et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission. Neurotoxicology. 2021 May;84:1 WB ; Rat.

PubMed:33549657

[IF=1.034] Yuyang Li. et al. Porphyromonas gingivalis lipopolysaccharide affects oral epithelial connections via pyroptosis. J Dent Sci. 2021 Jan;: IHC ; Human.

PubMed:10.1016/j.jds.2021.01.003

[IF=5.793] Yuan Changet al. NLRP3 inflammasome-mediated microglial pyroptosis is critically involved in the development of post-cardiac arrest brain injury. J Neuroinflammation . 2020 Jul 23;17(1):219. WB ; rat.

PubMed:32703306

[IF=4.527] Gao M et al. Is NLRP3 or NLRP6 inflammasome activation associated with inflammation-related lung tumorigenesis induced by benzo (a) pyrene and lipopolysaccharide? Ecotoxicol Environ Saf. 2019 Sep 24;185:109687. IHC ; Mouse.

PubMed:31561077

[IF=2.649] Duan S et al. NLRP3 inflammasome activation is associated with PM2. 5‐induced cardiac functional and pathological injury in mice. Environ Toxicol. 2019 Jul 16. IHC ; Mouse.

PubMed:31313453

[IF=3.553] Wu Z et al. Pyroptosis engagement and bladder urothelial cells derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after UPEC infection. Am J Physiol Cell Physiol. 2019 Jun 26. WB ; Mouse.

PubMed:31241987

[IF=3.063] Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245. WB ; broiler chick.

PubMed:29990747

[IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965 WB ; Duck.

PubMed:34775344

[IF=3.921] Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-κB signaling pathway. Neurochem Int. 2021 Nov;:105221 WB ; Rat.

PubMed:34780806

[IF=4.691] Xiaoli Liu. et al. Bone marrow mesenchymal stem cell-derived exosomes attenuate cerebral ischemia-reperfusion injury-induced neuroinflammation and pyroptosis by modulating microglia M1/M2 phenotypes. Exp Neurol. 2021 Mar;:113700 WB ; Rat.

PubMed:33741350

[IF=3.758] Lei Xianghong. et al. Micheliolide Attenuates Lipopolysaccharide-Induced Inflammation by Modulating the mROS/NF-κB/NLRP3 Axis in Renal Tubular Epithelial Cells. Mediat Inflamm. 2020;2020:3934769 WB ; Rat.

PubMed:32879619

[IF=3.943] Mengli Cui et al. NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury. Int Immunopharmacol. 2020 Jul;84:106492. WB ; Mouse.

PubMed:3482947

[IF=2.649] Shao H et al. Glyburide attenuates ozone‐induced pulmonary inflammation and injury by blocking the NLRP3 inflammasome. Environ Toxicol. 2020 Mar 13. IHC ; mouse.

PubMed:32167222

[IF=0] Kong D et al. IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury. Research Square. 17 February 2020. WB ; Rat.

PubMed:DOI:10.21203/rs.2.23662/v1

[IF=0.439] Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582. WB ; Rat.

PubMed:doi:10.4314/tjpr.v18i12.17

[IF=3.188] Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182. WB ; Rat.

PubMed:30890453