Rabbit Anti-Nogo R antibody

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NgR; Nogo-66 receptor; Nogor; NogoR; Nogo-R; Reticulon 4 receptor; Rtn4r; RTN4R_RAT.

Cat:

SL0129R

Species Reactivity：

Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from mouse Nogo R:151-350/473

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hippocampus (Rat) Lysate at 40 ugPrimary: Anti- Nogo R (SL0129R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 48 kDSample: Brain (Rat) Lysate at 40 ugPrimary: Anti- Nogo R (SL0129R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 48 kDSample: Hippocampus (Mouse) Lysate at 40 ugPrimary: Anti- Nogo R (SL0129R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 48 kDSample: Brain (Mouse) Lysate at 40 ugPrimary: Anti- Nogo R (SL0129R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 48 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Nogo-R Polyclonal Antibody, Unconjugated(SL0129R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Axons are essential for neuronal communication but they do not regenerate after injury to the adult mammalian brain or spinal cord. Failed regeneration is due in part to the production of a potent axonal growth inhibitor, Nogo, by myelinating cells. The finding of a high affinity axonal receptor for the extracellular domain of Nogo provides the first insight into the basis of Nogo action. Disrupting the interaction of Nogo with the Nogo-66 receptor may facilitate axonal regeneration in vivo. The protein is dubbed the Nogo receptor because it binds with several other proteins that block neural growth. It is found to be ubiquitous in the brain and spinal cord.

Function:
Receptor for RTN4, OMG and MAG. Mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. Acts in conjunction with RTN4 and LIGO1 in regulating neuronal precursor cell motility during cortical development (By similarity).

Subunit:
Homomultimer. Interacts with LINGO1. Interacts with KIAA0319L.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Similarity:
Belongs to the Nogo receptor family.
Contains 8 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.

SWISS:
Q99PI8

Gene ID:
65079

Database links:

Entrez Gene: 65078 Human

Entrez Gene: 65079 Mouse

Entrez Gene: 113912 Rat

Omim: 605566 Human

SwissProt: Q9BZR6 Human

SwissProt: Q99PI8 Mouse

SwissProt: Q99M75 Rat

Unigene: 30868 Human

Unigene: 40149 Mouse

Picture

Sample:
Brain (Rat) Lysate at 40 ug
Primary: Anti- Nogo R (SL0129R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- Nogo R (SL0129R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Nogo R (SL0129R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Nogo-R Polyclonal Antibody, Unconjugated(SL0129R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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