Rabbit Anti-MRP2/ABCC2 antibody

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multidrug resistance-associated protein2; ABC30; ABCC2; ATP binding cassette sub family C (CFTR/MRP) member 2; ATP binding cassette subfamily C member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; CMOA

Cat:

SL1092R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human MRP2:485-615/1545<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ugLane 2: A549 (Human) Cell Lysate at 30 ugLane 3: Panc-1 (Human) Cell Lysate at 30 ugPrimary: Anti-MRP2/ABCC2 (SL1092R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-ABCC2 (SL1092R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 174 kDObserved band size: 174 kDSample: HepG2(Human) Cell Lysate at 40 ugPrimary: Anti- MRP2/ABCC2 (SL1092R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 174 kDObserved band size: 174 kDTissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MRP2 Polyclonal Antibody, Unconjugated(SL1092R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human liver carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MRP2 Polyclonal Antibody, Unconjugated(SL1092R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. Blank control: A549. Primary Antibody (green line): Rabbit Anti-ABCC2 antibody (SL1092R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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multidrug resistance-associated protein 2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. Belongs to the ABC transporter family.

Function:
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.

DISEASE:
Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.

Similarity:
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.

SWISS:
Q92887

Gene ID:
1244

Database links:

Entrez Gene: 1244 Human

Entrez Gene: 12780 Mouse

Entrez Gene: 25303 Rat

Omim: 601107 Human

SwissProt: Q92887 Human

SwissProt: Q8VI47 Mouse

SwissProt: Q63120 Rat

Unigene: 368243 Human

Unigene: 39054 Mouse

Unigene: 10265 Rat

MRP-2作为一种结合输出泵，转运许多不同的药物结合物的蛋白。
MRP2蛋白的染色阳性产物分布于细胞浆/膜中。 Picture

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-ABCC2 (SL1092R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 174 kD
Observed band size: 174 kD

Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti- MRP2/ABCC2 (SL1092R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 174 kD
Observed band size: 174 kD

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MRP2 Polyclonal Antibody, Unconjugated(SL1092R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human liver carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MRP2 Polyclonal Antibody, Unconjugated(SL1092R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.

Blank control: A549.
Primary Antibody (green line): Rabbit Anti-ABCC2 antibody (SL1092R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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