Rabbit Anti-Survivin antibody

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API4; API-4; API 4; Survivin variant 3 alpha; Apoptosis Inhibitor 4; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; Baculoviral IAP repeat-containing protein 5; BIRC 5;

Cat:

SL0615R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human Survivin:85-142/142

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Small intestine (mouse) Lysate at 40 ugPrimary: Anti- Survivin (SL0615R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 16.5 kDObserved band size: 20 kDTissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Survivin Polyclonal Antibody, Unconjugated(SL0615R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human Gastric cancer cells; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Survivin Polyclonal Antibody, Unconjugated(SL0615R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Human oral squamous cell carcinoma;4% Paraformaldehyde-fixed; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-HPV16-E7 Polyclonal Antibody, FITC conjugated(SL4623R-FITC) 1:200, 37°C, 40min; The secondary antibody was Anti-Survivin Polyclonal Antibody, Cy3 conjugated (SL0615R-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiBlank control (blue line): U251 Primary Antibody (green line): Rabbit Anti-Survivin antibody (SL0615R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Interacts with tubulin. Inhibitor of caspase-3 and caspase-7. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly (By similarity). Belongs to the IAP family.

Function:
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Subunit:
Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPSLCbound state. The monomer protects cells against apoptosis more efficiently than the dimer. Only the dimeric form is capable of enhancing tubulin stability in cells. When phosphorylated, interacts with HBXIP; the resulting complex binds pro-CASP9, as well as active CASP9, but much less efficiently. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with JTB. Interacts with CDCA8 and INCENP; interaction is direct. Interacts with EVI5. Interacts with GTP-bound RAN in both the S and M phases of the cell cycle. Interacts with USP9X. Interacts with tubulin. Interacts with BIRC2/c-IAP1. The acetylated form at Lys-129 interacts with STAT3. The monomeric form deacetylated at Lys-129 interacts with XPO1/CRM1. The monomeric form interacts with XIAP/BIRC4. Both the dimeric and monomeric form can interact with DIABLO/SMAC. Interacts with BIRC6/bruce.

Subcellular Location:
Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.

Tissue Specificity:
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Post-translational modifications:
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities.
Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

Similarity:
Belongs to the IAP family.
Contains 1 BIR repeat.

SWISS:
O15392

Gene ID:
332

Database links:

Entrez Gene: 332 Human

Entrez Gene: 11799 Mouse

Entrez Gene: 12841 Rat

Omim: 603352 Human

SwissProt: O15392 Human

SwissProt: O70201 Mouse

SwissProt: Q9JHY7 Rat

Unigene: 514527 Human

Unigene: 8552 Mouse

Unigene: 54471 Rat

Survivin是目前发现的最强的凋亡抑制因子，存活蛋白是细胞凋亡抑制基因家族中的一个新成员，主要表达于细胞周期中的G2/M期，在许多恶性肿瘤组织中均有表达，主要用于各种恶性肿瘤的研究。
是细胞凋亡的一种抑制因子、参与细胞凋亡调控，可以用于各种恶性肿瘤的细胞凋亡的研究。Survivin具有肿瘤特异性，只表达于肿瘤和胚胎组织，且与肿瘤细胞的分化增殖及浸润转移密切相关. Picture

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Survivin Polyclonal Antibody, Unconjugated(SL0615R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human Gastric cancer cells;
Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Survivin Polyclonal Antibody, Unconjugated(SL0615R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Human oral squamous cell carcinoma;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-HPV16-E7 Polyclonal Antibody, FITC conjugated(SL4623R-FITC) 1:200, 37°C, 40min; The secondary antibody was Anti-Survivin Polyclonal Antibody, Cy3 conjugated (SL0615R-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Blank control (blue line): U251
Primary Antibody (green line): Rabbit Anti-Survivin antibody (SL0615R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (8) | SL0615R has been referenced in 8 publications.

[IF=0] Assaf, Hanan A. Survivin as a novel biomarker in the pathogenesis of acne vulgaris and its correlation to insulin-like growth factor-I. Diss. Faculty of Medicine, South Valley University. WB ; Human.

PubMed:27803511

[IF=1.889] Shian Liao. et al. Construction of autophagy prognostic signature and analysis of prospective molecular mechanisms in skin cutaneous melanoma patients. Medicine. 2021 Jun 4; 100(22): e26219 IHC ; Human.

PubMed:34087900

[IF=9.933] Xingyi Xu. et al. A Honeycomb-Like Bismuth/Manganese Oxide Nanoparticle with Mutual Reinforcement of Internal and External Response for Triple-Negative Breast Cancer Targeted Therapy. 2021 Jul 23 WB ; Human.

PubMed:34297897

[IF=2.886] Yusi Liu. et al. Synergistic Effects of Resveratrol and Temozolomide Against Glioblastoma Cells: Underlying Mechanism and Therapeutic Implications. Cancer Manag Res. 2020; 12: 8341–8354 ICC ; Rat, Human.

PubMed:32982428

[IF=2.558] Shi CT et al. High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma. J Breast Cancer. 2019 Apr 19;22(2):248-259. IHSLCP ; Human.

PubMed:31281727

[IF=1.871] Tong XZ et al. Survivin is a prognostic indicator in glioblastoma and may be a target of microRNA‑218. Oncology Letters.2019 doi:10.3892/ol.2019.10335 WB ; Human.

PubMed:DOI:10.3892/ol.2019.10335

[IF=2.564] Liu J et al. Survivin expression and localization in different organs of yaks (Bos grunniens). Gen Comp Endocrinol. 2018 Nov 1;268:80-87. WB ; yak.

PubMed:30077795

[IF=2.942] Hassan Mohammed H.. et al. Circulating and local nuclear expression of survivin and fibulin-3 genes in discriminating benign from malignant respiratory diseases: correlation analysis. Bioscience Rep. 2021 Jan;41(1):BSR20203097 WB ; Human.

PubMed:33226065