Rabbit Anti-TGF Beta 1 antibody

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CED; DPD1; LAP; Latency-associated peptide; TGFβ-1; TGFβ1; TGF beta; TGF-beta 1; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Gr

Cat:

SL0103R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthetic peptide derived from human TGF beta 1:101-150/390

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lymph node(Mouse) Lysate at 30 ugPrimary: Anti- TGF Beta 1(SL0103R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 54 kDSample: Bones(Mouse) Lysate at 30 ugPrimary: Anti- TGF Beta 1(SL0103R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 55 kDSample: K562 Cell Lysate at 40 ugPrimary: Anti- TGF Beta 1(SL0103R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 60 kDSample: Lane 1: K562 (Human) Cell Lysate at 30 ugLane 2: U2os (Human) Cell Lysate at 30 ugPrimary: Anti-TGF Beta 1 (SL0103R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50/35 kDObserved band size: 50/35 kDTissue/cell: human Pituitary tumor; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TGF-beta1 Polyclonal Antibody, Unconjugated(SL0103R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Oral squamous cell carcinoma;4% Paraformaldehyde-fixed; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TGF-beta1 Polyclonal Antibody, Unconjugated(SL0103R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE-Cy3 conjugated(SL0295G-PE-Cy3)used at 1:200 dilution for 40 minutes at 37°C. Excitation wavelength: 530nm; Emission wavelength:578nm

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]

Function:
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Subunit:
Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

Post-translational modifications:
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

DISEASE:
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

Similarity:
Belongs to the TGF-beta family.

SWISS:
P01137

Gene ID:
7040

Database links:

Entrez Gene: 7040 Human

Entrez Gene: 2363 Mouse

Entrez Gene: 59086 Rat

Omim: 19036 Human

SwissProt: P01137 Human

SwissProt: P04202 Mouse

SwissProt: P17246 Rat

Unigene: 645227 Human

Unigene: 248380 Mouse

Unigene: 40136 Rat

生长因子和激素（ Growth Factor and Hormones） TGF是一种多效生长因子，对上皮细胞增值有潜在抑制作用，可抑制肿瘤生长用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。 TGFβ超级家族由为数众多的生长的分化因子组成,包括转移生长因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盘生长因子(PTGF-β)；生长/分化因子(GDFs)；缪氏抑制物(MIS)；骨形态形成蛋白(BMPs)；交织细胞元神经生长因子(GDNF);抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超级家族成员参与胚胎发育和成体组织体内平衡。TGF-β1抗体与TGF-β2和TGF-β3没有交叉反应。与豚鼠有大部分交叉. Picture

Sample: Bones(Mouse) Lysate at 30 ug
Primary: Anti- TGF Beta 1(SL0103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 55 kD

Sample: K562 Cell Lysate at 40 ug
Primary: Anti- TGF Beta 1(SL0103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 60 kD

Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Primary:
Anti-TGF Beta 1 (SL0103R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/35 kD
Observed band size: 50/35 kD

Tissue/cell: human Pituitary tumor; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta1 Polyclonal Antibody, Unconjugated(SL0103R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Oral squamous cell carcinoma;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta1 Polyclonal Antibody, Unconjugated(SL0103R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE-Cy3 conjugated(SL0295G-PE-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Excitation wavelength: 530nm; Emission wavelength:578nm

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Specific References (19) | SL0103R has been referenced in 19 publications.

[IF=4.192] WB ; mouse.

PubMed:32275817

[IF=3.69] WB ; rat.

PubMed:32810620

[IF=8.947] Anqi Liu. et al. Core fucosylation involvement in the paracrine regulation of proteinuria-induced renal interstitial fibrosis evaluated with the use of a microfluidic chip. Acta Biomater. 2022 Feb;: IF ; Human.

PubMed:10.1016/j.actbio.2022.02.020

[IF=5.116] E Xiang. et al. Human umbilical cord-derived mesenchymal stem cells prevent the progression of early diabetic nephropathy through inhibiting inflammation and fibrosis. Stem Cell Res Ther. 2020 Dec;11(1):1-14 IHC ; Rat.

PubMed:32746936

[IF=0.92] Dan Panet al. Morphological Alteration and TGF-β1 Expression in Multifidus with Lumbar Disc Herniation. Indian J Orthop . 2020 Jul 29;54(Suppl 1):141-149. IHC, IF ; Human.

PubMed:32952922

[IF=5.546] Lina Wuet al. Involvement of miR‐27a‐3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress. J Cell Physiol . 2021 Feb;236(2):1454-1468. IHC ; mouse.

PubMed:32691413

[IF=4.192] Zhang Y et al. Ginsenoside Rg3 alleviates complete Freund's adjuvant-induced rheumatoid arthritis in mice by regulating CD4+ CD25+ Foxp3+ Treg cell. J Agric Food Chem . 2020 Apr 29;68(17):4893-4902. WB ; mouse.

PubMed:32275817

[IF=4.739] Yan J et al. Platelet‐derived microvesicles promote endothelial progenitor cell proliferation in intimal injury by delivering TGF‐β1. FEBS J. 2020 Mar 13 WB ; rat.

PubMed:32167224

[IF=3.06] Yan Y et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020 Jan 2;25(1). pii: E192. WB ; Human.

PubMed:31906574

[IF=3.923] Fang DP et al. Platelet‐rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF‐β/SMAD signaling pathway. J Cell Physiol. 2019;1–11. WB ; Rabbit.

PubMed:doi:10.1002/jcp.28211

[IF=3.457] Zhang L et al. Administration of methyl palmitate prevents non-alcoholic steatohepatitis (NASH) by induction of PPAR-α.(2018) Biomed. Pharmacother. 111 WB ; Mouse.

PubMed:30579258

[IF=2.608] Ji K et al. Differential Expression of lncRNAs and predicted target genes in normal mouse melanocytes and B16 cells. Experimental Dermatology. 2018. WB ; Mouse.

PubMed:10.1111/exd.13768

[IF=1.66] Wang et al. Effects of Tongxinluo on myocardial fibrosis in diabetic rats. (2016) J.Chin.Med.Assoc. 79:130-6 IHC ; Rat.

PubMed:26775602

[IF=1.69] Hu et al. Liraglutide attenuates the osteoblastic differentiation of MC3T3‑E1 cells by modulating AMPK/mTOR signaling. (2016) Mol.Med.Re. 14:3662-8 WB ; Mouse.

PubMed:27600753

[IF=2.479] Wang H et al. The effects of a hirudin/liposome complex on a diabetic nephropathy rat model. BMC Complement Altern Med. 2019 Jun 6;19(1):118. IHSLCP ; Rat.

PubMed:31170978

[IF=2.571] Yuanbo Cui. et al. HOXD1 functions as a novel tumor suppressor in kidney renal clear cell carcinoma. 2021 Feb 19 WB ; Human.

PubMed:33559901

[IF=1.813] Deng Xinqi. et al. Protective Effects and Metabolic Regulatory Mechanisms of Shenyan Fangshuai Recipe on Chronic Kidney Disease in Rats. Evid-Based Compl Alt. 2020;2020:5603243 IHC ; Rat.

PubMed:32908562

[IF=5.156] Liu, Tao. et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021 Dec;23(1):1-15 IHC ; Human.

PubMed:34433493

[IF=4.694] Qi SS et al. Protective Effects of Chromium Picolinate Against Diabetic-Induced Renal Dysfunction and Renal Fibrosis in Streptozotocin-Induced Diabetic Rats. Biomolecules. 2020 Mar 4;10(3). IHC ; rat.

PubMed:32143429