Rabbit Anti-VEGFA antibody | Sunlong Medical

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Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; VEGF-A; Vascular permeability factor (VPF);

Cat:

SL0279R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthetic peptide derived from human VEGF:27-120/232

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart(Mouse) Lysate at 30 ugPrimary: Anti-VEGF (SL0279R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 24 kDObserved band size: 25 kDSample: Kidney(Mouse) Lysate at 30 ugPrimary: Anti-VEGF (SL0279R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 24 kDObserved band size: 25 kDSample: heart (Mouse) Lysate at 40 ugA549 Cell (Human) Lysate at 30 ugPrimary: Anti- VEGF (SL0279R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 24 kDObserved band size: 24 kDTissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(SL0279R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingCell: HelaConcentration:1:100Host/Isotype:Rabbit/IgGFlow cytometric analysis of Rabbit antibody (Cat#: SL0279R) on Hela(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq].

Function:
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Subunit:
Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Subcellular Location:
Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

Tissue Specificity:
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

DISEASE:
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

SWISS:
P15692

Gene ID:
7422

Database links:

Entrez Gene: 7422 Human

Entrez Gene: 22339 Mouse

Entrez Gene: 83785 Rat

Omim: 19248 Human

SwissProt: P15692 Human

SwissProt: Q00731 Mouse

SwissProt: P16612 Rat

Unigene: 73793 Human

Unigene: 282184 Mouse

Unigene: 1923 Rat

生长因子和激素(Growth Factor and Hormones) 血管内皮细胞生长因子(VEGF)是一种特异作用于血管内皮细胞的多功能细胞因子,它能引起血管通透性增加,引起细胞外基质成分改变,诱导血管形成.在炎症、创伤愈合、心脏缺血、动脉粥样硬化、糖尿病性视网膜病变及肿瘤形成等与血管生成和病变有关的诸多病理过程中起重要作用.
VEGF与血管生成有关，从而也与肿瘤生长也有很大关系,近年来受到很多关注. Picture

Sample: Heart(Mouse) Lysate at 30 ug
Primary: Anti-VEGF (SL0279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 25 kD

Sample: Kidney(Mouse) Lysate at 30 ug
Primary: Anti-VEGF (SL0279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 25 kD

Sample:
heart (Mouse) Lysate at 40 ug
A549 Cell (Human) Lysate at 30 ug
Primary: Anti- VEGF (SL0279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD

Tissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(SL0279R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Cell: Hela
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit antibody (Cat#: SL0279R) on Hela(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

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Specific References (29) | SL0279R has been referenced in 29 publications.

[IF=2.72] Jiebin Li. et al. Targeted Temperature Management Suppresses Hypoxia-Inducible Factor-1α and Vascular Endothelial Growth Factor Expression in a Pig Model of Cardiac Arrest. 2021 Jan 05 WB ; Pig.

PubMed:33403582

[IF=5.256] Wang J et al. A Novel RNA Aptamer-modified Riboswitch as Chemical sensor. Analytica Chimica Acta. (2019). Other ;

PubMed:doi:10.1016/j.aca.2019.11.071

[IF=2.467] Zheng W et al. An injectable thermosensitive hydrogel for sustained release of apelin-13 to enhance flap survival in rat random skin flap. J Mater Sci Mater Med. 2019 Sep 9;30(9):106. IHC&WB ; Rat.

PubMed:31502009

[IF=5.646] Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55. FCM&IHC ; Mouse&Human.

PubMed:30728051

[IF=2.064] Yao Q et al.Lycium Barbarum Polysaccharides Improve Retinopathy in Diabetic Sprague-Dawley Rats.(2018)Evid Based Complement Alternat Med.Nov 15;2018:7943212. WB ; Rat.

PubMed:30581486

[IF=1.664] Nie M et al. Correlation between ultrasonic features and expression levels of SLCerbB-2, VEGF and nm23 in breast cancer.Oncol Lett. 2018 Aug;16(2):1701-1707. IHC ; Human.

PubMed:30008856

[IF=4.26] Bi et al. Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice. (2017) Sci.Rep. 7:10001 IHC ; Mouse.

PubMed:28855642

[IF=1.41] Chen et al. Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair. (2018) Exp.Ther.Med. 16:821-829 IHC ; Mouse.

PubMed:30112037

[IF=3.14] Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017). WB ; Human.

PubMed:28552725

[IF=3.41] Qi, Yuanyuan, et al. "Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells." PloS one 7.3 (2012): e33859. WB ; Pig.

PubMed:22448277

[IF=1] Guo, Jian-wen, et al. "Combinatorial effects of Naomai Yihao Capsules () and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tissues in rats." Journal of Traditional Chinese Medicine 32.1 (2012): 87-92. IHSLCP ; Rat.

PubMed:22594109

[IF=3.571] Mei F et al. Collagen peptides isolated from Salmon salar and Tilapia nilotica skin accelerate wound healing by altering cutaneous microbiome colonization via up-regulated NOD2 and BD14. Agric. Food Chem. 2020, 68, 6, 1621-1633. IHSLCP ; Rat.

PubMed:doi:10.1021/acs.jafc.9b08002

[IF=0] Hub Y et al. Biomimetic fabrication of Icariin loaded nano hydroxyapatite reinforced bioactive porous scaffolds for bone regeneration. Chemical Engineering Journal, 394, 124895. IHC ; rat.

PubMed:10.1016/j.cej.2020.124895

[IF=3.375] Zheng Chen. et al. Glycine attenuates cerebrovascular remodeling via glycine receptor alpha 2 and vascular endothelial growth factor receptor 2 after stroke. Am J Transl Res. 2020; 12(10): 6895–6907 IF,IHC ; Rat.

PubMed:33194080

[IF=2.172] Yuchen Yao. et al. Seasonal expressions of VEGF and its receptors VEGFR1 and VEGFR2 in the prostate of the wild ground squirrels (Spermophilus dauricus). Eur J Histochem. 2021 Mar 24; 65(2): 3219 IHC ; squirrel.

PubMed:33712818

[IF=2.842] Guangrui Chai. et al. NLRP3 Blockade Suppresses Pro-Inflammatory and Pro-Angiogenic Cytokine Secretion in Diabetic Retinopathy. Diabet Metab Synd Ob. 2020; 13: 3047–3058 WB ; Human.

PubMed:32904641

[IF=5.572] Siddharth J. Modi. et al. Discovery of VEGFR-2 inhibitors exerting significant anticancer activity against CD44+ and CD133+ cancer stem cells (CSCs): Reversal of TGF-β induced epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma. Eur J Med Chem. 2020 Dec;207:112851

PubMed:33002846

[IF=1.624] Kun Wuet al. Preparation and evaluation of heparinized sponge based on collagen and chitosan for wound healing. Journal Of Bioactive And Compatible Polymers IHC ; mouse.

PubMed:10.1177/0883911520939983

[IF=3.332] Sun Y et al. The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism. J Cancer Res Clin Oncol. 2020 May;146(5):1139-1152. IHC,WB ; human.

PubMed:32152662

[IF=0.17] W Jiang et al. EFFECTS OF MESENCHYMAL STEM CELL TRANSPLANTATION INTO ISCHEMIC BILE DUCT INJURY TISSUES OF RATS ON THE EXPRESSION OF VASCULAR DENSITY. Slov Vet Res 2019: 56 (4): 183–90. IHSLCP ; Rat.

PubMed:DOI:10.26873/SVR-844-2019

[IF=3.361] Varghese S et al. Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Int Immunopharmacol. 2019 May 21;73:280-292. WB ; Human.

PubMed:31125927

[IF=2.219] Fernandez-Bueno I et al. Reliability and reproducibility of a rodent model of choroidal neovascularization based on the subretinal injection of polyethylene glycol.Mol Vis. 2019 Mar 18;25:194-203. IHSLCP ; Mouse.

PubMed:30996588

[IF=3.923] Yang Y et al. Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR. (2018) J Cell Physiol. WB ; Mouse.

PubMed:30515795

[IF=1.77] Wang et al. Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage. (2012) Neural.Regen.Res. 7:595-600 IHSLCF ; Rat.

PubMed:25745450

[IF=4.938] Chen et al. Circulating fibrocyte mobilization in negative pressure wound therapy. (2017) J.Cell.Mol.Me. 21:1513-1522 WB ; Rat.

PubMed:28211211

[IF=3.89] Ryzhov, Sergey, et al. "Role of a2b adenosine receptors in regulation of paracrine functions of stem cell antigen 1-positive cardiac stromal cells." Journal of Pharmacology and Experimental Therapeutics 341.3 (2012): 764-774. Mouse.

PubMed:22431204

[IF=0.78] Zhang, Q. L., et al. "Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of liaoning cashmere goats." Genet Mol Res 12.12 (2013): 6424-6432. IHSLCP ; Goat.

PubMed:24390991

[IF=3.17] Li, Junqin, et al. "Fatty Acid Synthase Mediates the Epithelial-Mesenchymal Transition of Breast Cancer Cells." International Journal of Biological Sciences 10.2 (2014): 171-36. WB ; Human.

PubMed:24520215

[IF=2.59] Zeng, K.-W., et al., Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis. Eur J Pharmacol (2013) Mouse.

PubMed:23684542