Rabbit Anti-IL12 antibody | Sunlong Medical

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Interleukin-12 subunit alpha; IL-12; CLMF p35; CLMF1; CTL maturation factor (TcMF); Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL 12 subunit p35; IL12A; Interleukin 12 alpha chain; Interleukin 12 p35;

Cat:

SL0767R

Species Reactivity：

Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from mouse IL-12:51-150/215

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugNIH/3T3 (Mouse) CellLysate at 30 ugRAW246.7 (Mouse) CellLysate at 30 ugPrimary: Anti- IL12 (SL0767R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 35/36 kDSample: Lane 1: Spleen (Mouse) Lysate at 40 ugLane 2: Blood cell (Mouse) Lysate at 40 ugLane 3: Raw264.7 (Mouse) Cell Lysate at 30 ugLane 4: Liver (Mouse) Lysate at 40 ugLane 5: Spleen (Rat) Lysate at 40 ugLane 6: Liver (Rat) Lysate at 40 ugPrimary: Anti-IL12 (SL0767R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 33 kDObserved band size: 35 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (SL0767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (SL0767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated(SL0767R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control (blue line): Mouse spleen (blue). Primary Antibody (green line): Rabbit Anti- IL12 antibody (SL0767R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Function:
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Subunit:
Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.

Subcellular Location:
Secreted.

Similarity:
Belongs to the IL-6 superfamily.

SWISS:
P43431

Gene ID:
16159

Database links:

Entrez Gene: 3592 Human

Entrez Gene: 16159 Mouse

Entrez Gene: 8885 Rat

Omim: 161560 Human

SwissProt: P29459 Human

SwissProt: P43431 Mouse

SwissProt: Q9R103 Rat

Unigene: 673 Human

Unigene: 103783 Mouse

Unigene: 207199 Rat

IL-12是新近发现的细胞因子，具有多种生物学活性，尤其是在抗肿瘤免疫和抗病毒免疫中有重要的作用。 Picture

Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Blood cell (Mouse) Lysate at 40 ug
Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 4: Liver (Mouse) Lysate at 40 ug
Lane 5: Spleen (Rat) Lysate at 40 ug
Lane 6: Liver (Rat) Lysate at 40 ug
Primary:
Anti-IL12 (SL0767R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 35 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (SL0767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (SL0767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated(SL0767R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody (SL0767R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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