Rabbit Anti-CD28 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-CD28 antibody

CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44; CD28_HUMAN; CD28_MOUSE.

Cat:

SL1297R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse CD28:1-120/218<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ugLane 2: Thymus (Mouse) Lysate at 40 ugPrimary: Anti-CD28 (SL1297R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: Thymus (Rat) Lysate at 40 ugPrimary: Anti-CD28 (SL1297R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 44kDParaformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (SL1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(SL1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(SL1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingPositive control: (mo)Splenocytes(2% Paraformaldehyde-fixed )Isotype Control AntibodyAntibody: Rabbit IgG; Supplier: Cell Signaling Technology; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSASecondary AntibodyAntibody: Goat anti-rabbit IgG-FITC;Supplier: Cell Signaling Technology;Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary AntibodySupplier: Bioss; Supplier catalog number: SL1297R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Function:
Possibly involved in T-cell activation.

Subunit:
Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
P31041

Gene ID:
12487

Database links:

Entrez Gene: 940 Human

Entrez Gene: 12487 Mouse

Omim: 186760 Human

SwissProt: P10747 Human

SwissProt: P31041 Mouse

SwissProt: P42069 Rabbit

Unigene: 591629 Human

Unigene: 255003 Mouse

CD28是免疫球蛋白超家族成员之一，为T细胞特异性由二硫键连接的同源二聚体表面糖蛋白，主要分布在外周血淋巴细胞。对迟发型超敏反应以及Tc介导的细胞毒作用都有免疫调节作用。 Picture

Sample:
Thymus (Rat) Lysate at 40 ug
Primary: Anti-CD28 (SL1297R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 44kD

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (SL1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min;
Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(SL1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min;
Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(SL1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody
Antibody: Rabbit IgG; Supplier: Cell Signaling Technology; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC; Supplier: Cell Signaling Technology; Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier: Bioss; Supplier catalog number: SL1297R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

Product Feedback Wall