Rabbit Anti-CD8 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-CD8 antibody

CD8 antigen, alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymph

Cat:

SL0648R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthetic peptide derived from mouse CD8:51-150/247<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: mCD8a overexpression 293T Lysate at 8 ug Primary: Anti-CD8a (SL0648R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35-42 kD Observed band size: 41 kDSample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti- CD8 (SL0648R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 36 kDSample:Lymph node(Mouse) Lysate at 30 ugPrimary: Anti-CD8(SL0648R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 32 kDImage kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (SL0648R) at 1:250.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].

Function:
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit:
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
P01731

Gene ID:
12525

Database links:

Entrez Gene: 925 Human

Entrez Gene: 12525 Mouse

SwissProt: P01732 Human

SwissProt: P01731 Mouse

Picture

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- CD8 (SL0648R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 36 kD

Sample:Lymph node(Mouse) Lysate at 30 ug
Primary: Anti-CD8(SL0648R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 32 kD

Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (SL0648R) at 1:250.

Product Feedback Wall

Specific References (57) | SL0648R has been referenced in 57 publications.

[IF=2.37] Qiao, Xinwei, et al. "Increased expression of Tim-3 and its ligand Galectin-9 in rat allografts during acute rejection episodes." Biochemical and biophysical research communications 445.3 (2014): 542-548. IHSLCF ; Rat.

PubMed:24508263

[IF=4.556] Noritaka Yonezawa. et al. Abscopal Effect of Frozen Autograft Reconstruction Combined with an Immune Checkpoint Inhibitor Analyzed Using a Metastatic Bone Tumor Model. Int J Mol Sci. 2021 Jan;22(4):1973 IHC ; Mouse.

PubMed:33671258

[IF=8.128] Xinwei Kuang. et al. Ag nanoparticles enhance immune checkpoint blockade efficacy by promoting of immune surveillance in melanoma. J Colloid Interf Sci. 2022 Jun;616:189 IF ; Mouse.

PubMed:10.1016/j.jcis.2022.02.050

[IF=13.281] Yao Li. et al. Antigen Capture and Immune Modulation by Bacterial Outer Membrane Vesicles as In Situ Vaccine for Cancer Immunotherapy Post-Photothermal Therapy. 2022 Feb 12 IF ; Mouse.

PubMed:35152555

[IF=6.081] Long-Yi Chan. et al. Cre/LoxP Genetic Recombination Sustains Cartilage Anabolic Factor Expression in Hyaluronan Encapsulated MSCs Alleviates Intervertebral Disc Degeneration. Biomedicines. 2022 Mar;10(3):555 IHC ; Rat.

PubMed:10.3390/biomedicines10030555

[IF=10.479] Christophe Glorieux. et al. Cisplatin and gemcitabine exert opposite effects on immunotherapy with PD-1 antibody in K-ras-driven cancer. J Adv Res. 2021 Dec;: IHC ; Human,Mouse.

PubMed:10.1016/j.jare.2021.12.005

[IF=3.685] Weili Xu. et al. Targeted elimination of myeloid-derived suppressor cells via regulation of the STAT pathway alleviates tumor immunosuppression in neuroblastoma. Immunol Lett. 2021 Dec;48:31 IF ; Mouse.

PubMed:3920949

[IF=10.435] Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26 IF ; Mouse.

PubMed:34715867

[IF=7.182] Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211 IF ; Mouse.

PubMed:34127215

[IF=2.614] Muhammad Ishfaq. et al. Baicalin attenuated Mycoplasma gallisepticum‐induced immune impairment in chicken bursa of fabricius through modulation of autophagy and inhibited inflammation and apoptosis. J Sci Food Agr. 2021 Feb;101(3):176-890 IF ; Chicken.

PubMed:32729138

[IF=7.367] Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28 IF,IHC ; Mouse.

PubMed:33644993

[IF=7.068] Wenli Fang. et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 + T cell exclusion. J Exp Clin Canc Res. 2021 Dec;40(1):1-11 IF ; Mouse.

PubMed:33390170

[IF=3.943] Yihan Lu et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563. IF ; Mouse.

PubMed:32442899

[IF=4.213] Wan WJ et al. Doxorubicin and siRNA-PD-L1 co-delivery with T7 modified ROS-sensitive nanoparticles for tumor chemoimmunotherapy. Int J Pharm. 2019 Jul 20;566:731-744. IHF ; Mouse.

PubMed:31212055

[IF=2.976] Peng Y et al. Sonodynamic therapy improves anti‑tumor immune effect by increasing the infiltration of CD8+ T cells and altering tumor blood vessels in murine B16F10 melanoma xenograft. Oncol Rep. 2018 Oct;40(4):2163-2170. IHF-P&WB ; Mouse.

PubMed:30106435

[IF=8.806] Li TF et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.Biomaterials. 2018 Oct;181:35-52. IHSLCP ; Human.

PubMed:3007276

[IF=1.26] Jaime-Ramirez et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. (2017) Mol.Ther.Oncolytics. 5:87-96 IHSLCP ; Mouse.

PubMed:28812060

[IF=7.786] Zhu,et al.Co-delivery of tumor antigen and dual toll-like receptor ligands into dendritic cell by silicon microparticle enables efficient immunotherapy against melanoma.(2018) Journal of Controlled Release. 272:72-82. IHSLCP ; Mouse.

PubMed:29325699

[IF=2.485] Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31 IHC ; Mouse.

PubMed:27669687

[IF=6.493] Ashraf et al. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation. (2016) Am.J.Transplant. 16:808-20 IHSLCP ; Mouse.

PubMed:26595644

[IF=2.885] Schmid et al. Acute Renal Graft-Versus-Host Disease in a Murine Model of Allogeneic Bone Marrow Transplantation. (2017) Cell.Transplant. 26:1428-188 IHC ; Mouse.

PubMed:28901194

[IF=2.232] Dong et al. Antitumor effect of embryonic stem cells in a non-small cell lung cancer model: antitumor factors and immune responses. (2013) Int.J.Med.Sc. 10:1314-20 IHSLCP ; Mouse.

PubMed:23983591

[IF=4] Prystaz, Katja, et al. "Distinct effects of interleukin-6 classic and trans-signaling in bone fracture healing." The American Journal of Pathology. IHSLCP ; Mouse.

PubMed:29146294

[IF=3.03] Willett, Emily S., et al. "Standardized Rat Model Testing Effects of Inflammation and Grafting on Extraction Healing." Journal of Periodontology (2017): 1-15. IHC ; Rat.

PubMed:2888741

[IF=3.26] Tanaka, Rui, et al. "The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model." American Journal of Cancer Research 7.8 (2017): 1693. IHSLCP ; Mouse.

PubMed:28861325

[IF=1.99] Chuang, Meng-Hsien, et al. "The Chinese medicine JSLC001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response." BMC Complementary and Alternative Medicine 17.1 (2017): 210. IHSLCP ; Mouse.

PubMed:28399860

[IF=8.39] Wang, Raymond M., et al. "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." Biomaterials (2017). IHC ; Mouse.

PubMed:28334641

[IF=5.68] Ashraf, M. I., et al. "Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation." American Journal of Transplantation (2015). IHSLCP ; Mouse.

PubMed:26595644

[IF=1.69] Jeong, Keun-Yeong, et al. "Combination of macrophage inflammatory protein 1 alpha with existing therapies to enhance the antitumor effects on murine hepatoma." Journal of Radiation Research (2014): rru077. WB ; Mouse.

PubMed:25225286

[IF=3.061] Wang,et al.Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway.(2018) American Journal of Translational Research. 10:114-125. WB ; Human.

PubMed:29422998

[IF=4.069] Mödinger Y et al. Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing. (2018) Am J Pathol. Oct 17. IHC ; Mouse.

PubMed:30339839

[IF=3.285] Zhang H et al. Utilizing VEGF165b mutant as an effective immunization adjunct to augment antitumor immune response.Vaccine. 2019 Apr 3;37(15):2090-2098. IHSLCP ; Mouse.

PubMed:30837171

[IF=2.882] Gul Fatma Yarim. et al. Nobiletin attenuates inflammation via modulating proinflammatory and antiinflammatory cytokine expressions in an autoimmune encephalomyelitis mouse model. Fitoterapia. 2022 Jan;156:105099 IHC ; Rat.

PubMed:34896483

[IF=3.64] He, Qiushan, et al. "Low-dose paclitaxel enhances the anti-tumor efficacy of GM-CSF surface-modified whole-tumor-cell vaccine in mouse model of prostate cancer." Cancer Immunology, Immunotherapy 60.5 (2011): 715-730. IHSLCP ; Mouse.

PubMed:21331814

[IF=3.272] Miss Hongfang Meng. et al. Overexpression of hepatocyte growth factor in dental pulp stem cells ameliorates the severity of psoriasis by reducing inflammatory responses. 2021 Jun 22 IHC ; Mouse.

PubMed:34155928

[IF=7.727] Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306 IHC ; Mouse.

PubMed:34081995

[IF=29.497] Di Zhao. et al. Chromatin Regulator CHD1 Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer. Cancer Discov. 2020 Sep;10(9):1374-1387 IF,IHC ; Human.

PubMed:32385075

[IF=9.986] Christophe Glorieux. et al. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling. Redox Biol. 2021 Jan;38:10356 IHC ; Mouse.

PubMed:33171331

[IF=3.484] Boon Ching Teeet al. Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration. Xenotransplantation . 2020 Sep;27(5):e12625. IHC ; rat.

PubMed:32629548

[IF=16.907] Senbiao Chenaet al. Carrier-free nanoassembly of doxorubicin prodrug and siRNA for combinationally inducing immunogenic cell death and reversing immunosuppression. Nano Today 35 (2020) 100924 IHC ; mouse.

PubMed:10.1016/j.nantod.2020.100924

[IF=4.225] Zhao B et al. A TCM Formula YYWY Inhibits Tumor Growth in Non-Small Cell Lung Cancer and Enhances Immune-Response Through Facilitating the Maturation of Dendritic Cells. Front Pharmacol. 2020 Jun 9;11:798. IHF-P ; Mouse.

PubMed:32595493

[IF=4.658] Zhang Y et al. Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway. J Cell Mol Med. 2019 Nov 15. IHF-F ; Mouse.

PubMed:3172936

[IF=5.345] Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110. IHSLCP ; Mouse.

PubMed:31623629

[IF=4.982] Demontoux L et al. Hypotonic stress enhances colon cancer cell death induced by platinum derivatives and immunologically improves anti‐tumor efficacy of intraperitoneal chemotherapy. Int J Cancer. 2019 Jul 25. IHSLCP ; Mouse.

PubMed:31344262

[IF=1.655] Takamori S et al. HMGB1 blockade significantly improves luminal fibrous obliteration in a murine model of bronchiolitis obliterans syndrome.Takamori S et al. IHSLCP ; Mouse.

PubMed:30508580

[IF=1.448] Sun J1 et al. Clinical effects of lentinan combined with budesonide inhalation in treating acute exacerbation of chronic obstructive pulmonary disease under mechanical ventilation. Exp Ther Med. 2019 Mar;17(3):1503-1508. FCM ; Human.

PubMed:30867684

[IF=5.595] Rapp et al. Induced global deletion of glucocorticoid receptor impairs fracture healing. (2018) FASEB.J. 32:2235-2245 IHC ; Mouse.

PubMed:29217668

[IF=4.539] Kanai K et al. Murine γ-Herpesvirus 68 Induces Severe Lung Inflammation in IL-27-Deficient Mice with Liver Dysfunction Preventable by Oral Neomycin. J Immunol. 2018 Apr 15;200(8):2703-2713. IHSLCP ; Mouse.

PubMed:2950048

[IF=5.5] Simanovich et al. Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation. (2017) Oncoimmunology. 6:e1261778 IHC ; Mouse.

PubMed:28197388

[IF=4.2] Kovtun, Anna, et al. "Complement receptors C5aR1 and C5aR2 act differentially during the early immune response after bone fracture but are similarly involved in bone repair." Scientific Reports 7.1 (2017): 14061. IHSLCP ; Mouse.

PubMed:29070810

[IF=4.929] Wang et al. Targeted Disruption of the β2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. (2015) Stem.Cells.Transl.Me. 4:1234-45 IF(ICC) ; Human.

PubMed:26285657

[IF=1.41] Haffner-Luntzer, Melanie, et al. "The inflammatory phase of fracture healing is influenced by oestrogen status in mice." European Journal of Medical Research 22.1 (2017): 23. FCM ; Mouse.

PubMed:28683813

[IF=6.28] Kroner, Jochen, et al. "Mast cells are critical regulators of bone fracture‐induced inflammation and osteoclast formation and activity." Journal of Bone and Mineral Research (2017). IHSLCP ; Mouse.

PubMed:28777474

[IF=3.06] Zhang, Wen, et al. "The role of CXCR3 in the induction of primary biliary cirrhosis." Clinical and Developmental Immunology 2011 (2011). IHSLCP ; Mouse.

PubMed:21647407

[IF=4.56] Kovtun, A., et al. "The crucial role of neutrophil granulocytes in bone fracture healing." European Cells and Materials 32 (2016):152-162. IHSLCP ; Mouse.

PubMed:27452963

[IF=3.69] Li, Yan, et al. "Immunogenic FEAT protein circulates in the bloodstream of cancer patients." Journal of Translational Medicine 14.1 (2016): 275. IHSLCP ; Mouse.

Service
- Custom ELISA kit
About Us
- About Us
Resources
- Tech Feature
- Document Download
Contact Us
- Sunlong Medical
- Tel: [email protected]
- Email: [email protected]
Social Media