Rabbit Anti-Beclin 1 antibody

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Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.

Cat:

SL1353R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human BECN1:201-330/450

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Small intestine(Mouse) lysate at 30ug; Primary: Anti-Beclin-1 (SL1353R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 50kDObserved band size : 50kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (SL1353R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37鈩� for 20 min; Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(SL1353R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (SL1353R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (SL1353R ) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell: rat stomach tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(SL1353R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiTissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(SL1353R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.

Function:
Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Subunit:
Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB. Interacts with DAPK1. Interacts with human cytomegalovirus/HHSLV5 protein TRS1.

Subcellular Location:
Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.
Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.

Similarity:
Belongs to the beclin family.

SWISS:
Q14457

Gene ID:
8678

Database links:

Entrez Gene: 8678 Human

Entrez Gene: 56208 Mouse

Entrez Gene: 114558 Rat

Omim: 604378 Human

SwissProt: Q14457 Human

SwissProt: O88597 Mouse

SwissProt: Q91XJ1 Rat

Unigene: 716464 Human

Unigene: 178947 Mouse

Unigene: 2776 Rat

BECN1是一种分子质量为60KDa的蛋白质, 它能与细胞凋亡抑制剂Bcl-2相互作用, 减少中枢神经系统的凋亡，参与PI3K复合物的组成和自噬体的形成。主要表达在胞质。抑癌基因beclin 1通过自噬作用来调节细胞生长。 Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (SL1353R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37鈩� for 20 min;
Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(SL1353R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (SL1353R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (SL1353R ) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Tissue/cell: rat stomach tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(SL1353R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(SL1353R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Specific References (29) | SL1353R has been referenced in 29 publications.

[IF=4.3] Wang, Jinheng, et al. "Bcl-3, induced by Tax and HTLSLV1, inhibits NF-κB activation and promotes autophagy." Cellular Signalling (2013). WB ; Human.

PubMed:4844922

[IF=16.016] Jilong Zhou. et al. ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation. 2022 Mar 20 WB ; Mouse.

PubMed:3531192

[IF=5.81] Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784 WB,IHC ; Mouse.

PubMed:35308251

[IF=2.571] Wang L et al. 1‐(4‐((5‐chloro‐4‐((2‐(isopropylsulfonyl)phenyl)amino)pyrimidin‐2‐yl)amino)‐3‐methoxyphenyl)‐3‐(2‐(dimethylamino)ethyl)imidazolidin‐2‐one (ZX‐42), a novel ALK inhibitor, induces apoptosis and protective autophagy in H2228 cells. J Pharm Pharmacol. 2020 Oct;72(10):1370-1382. WB ; Human.

PubMed:32591369

[IF=4.932] Ibitamuno Caleb. et al. Characterizing Autophagy in the Cold Ischemic Injury of Small Bowel Grafts: Evidence from Rat Jejunum. Metabolites. 2021 Jun;11(6):396 IHC ; Rat.

PubMed:34204418

[IF=5.75] Liu P. et al. Scalp Acupuncture Attenuates Brain Damage After Intracerebral Hemorrhage Through Enhanced Mitophagy and Reduced Apoptosis in Rats.. Front Aging Neurosci. 2021 Dec;13:718631-718631 WB,IHC ; Rat.

PubMed:34987374

[IF=5.923] Hyoung Moon Kim. et al. Evaluating Whether Radiofrequency Irradiation Attenuated USLVB-Induced Skin Pigmentation by Increasing Melanosomal Autophagy and Decreasing Melanin Synthesis. Int J Mol Sci. 2021 Jan;22(19):10724 WB ; Mouse.

PubMed:34639063

[IF=4.432] Jinni Meng. et al. Dehydrocostuslactone attenuated oxygen and glucose deprivation/reperfusion-induced PC12 cell injury through inhibition of apoptosis and autophagy by activating the PI3K/AKT/mTOR pathway. Eur J Pharmacol. 2021 Nov;911:174554 WB ; Rat.

PubMed:34627804

[IF=4.432] Wei Jiang. et al. Caveolin-1 attenuates acetaminophen aggravated lipid accumulation in alcoholic fatty liver by activating mitophagy via the Pink-1/Parkin pathway. Eur J Pharmacol. 2021 Oct;908:174324 WB ; Mouse, Human.

PubMed:34246650

[IF=1.698] Sheng-Tao Ling. et al. Hydroxychloroquine Blocks Autophagy and Promotes Apoptosis of the Prostate after Castration in Rats. Urol Int. 2020;104(11-12):968-974 IHC ; Rat.

PubMed:32937616

[IF=3.067] Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. WB ; Mouse.

PubMed:3149296

[IF=3.04] Shi C et al. Acetaminophen aggravates fat accumulation in NAFLD by inhibiting autophagy via the AMPK/mTOR pathway. European Journal of Pharmacology.2019. WB ; Mouse&Human.

PubMed:10.1016/j.ejphar.2019.02.005

[IF=5.23] Chen, Cheng-Hsien, et al. "Far-infrared protects vascular endothelial cells from advanced glycation end products-induced injury via PLZF-mediated autophagy in diabetic mice." Scientific Reports 7 (2017): 40442. IHSLCP ; Mouse.

PubMed:28071754

[IF=1.482] Tian, Jing, Rong Liu, and Quanxin Qu. "Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma." Oncology Letters. WB ; Human.

PubMed:28454274

[IF=1.5] Wang, Fang-Li Ma, Ning Tao, and Zhi-Hai Qin. "Polysaccharide from Polygonatum Inhibits the Proliferation of Prostate Cancer-Associated Fibroblasts Cells." Asian Pacific Journal of Cancer Prevention 17.8 (2016): 3827-3831. WB ; Human.

PubMed:27644624

[IF=2.78] Chen, Yunbo, et al. "β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." Brain research 1552 (2014): 41-54. WB ; Mouse.

PubMed:24457043

[IF=3.23] Sun, Qianqian, et al. "Factors that Affect Pancreatic Islet Cell Autophagy in Adult Rats: Evaluation of a Calorie-Restricted Diet and a High-Fat Diet."PLOS ONE 11.3 (2016): e0151104. IHSLCP ; Rat.

PubMed:26963814

[IF=0.18] Liu, B., et al. "Autophagy activation aggravates neuronal injury in the hippocampus of vascular dementia rats." Neural Regeneration Research 9.13 (2014): 1288. IHSLCP ; Rat.

PubMed:25221581

[IF=5.64] Chengcheng Zhang. et al. Autophagy Induced by the N-Terminus of the Classic Swine Fever Virus Nonstructural Protein 5A Protein Promotes Viral Replication. Front Microbiol. 2021; 12: 733385 WB ; Pig.

PubMed:34512612

[IF=4.939] Tong-Fei Li. et al. Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma. Mol Pharmaceut. 2021;XXXX(XXX):XXX-XXX WB ; Pig.

PubMed:34388342

[IF=7.086] Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625 IF ; Mouse.

PubMed:34303901

[IF=3.263] Fengge Shen. et al. Aloe-emodin induces autophagy and apoptotic cell death in non-small cell lung cancer cells via Akt/mTOR and MAPK signaling. Eur J Pharmacol. 2020 Nov;886:173550 WB ; Human.

PubMed:32926915

[IF=2.1] Rong Hu. et al. Apatinib sensitizes chemoresistant NSCLC cells to doxetaxel via regulating autophagy and enhances the therapeutic efficacy in advanced and refractory/recurrent NSCLC. Mol Med Rep. 2020 Nov;22(5):3935-3943 WB ; Human.

PubMed:32901884

[IF=2.571] Lijing Wanget al. 1-(4-((5-chloro-4-((2-(isopropylsulfonyl)phenyl)amino)pyrimidin-2-yl)amino)-3-methoxyphenyl)-3-(2-(dimethylamino)ethyl)imidazolidin-2-one (ZX-42), a novel ALK inhibitor, induces apoptosis and protective autophagy in H2228 cells. J Pharm Pharmacol . 2020 Oct;72(10):1370-1382. WB ; Human.

PubMed:32591369

[IF=3.216] Lijuan Gao # et al. Chlorogenic Acid Alleviates Aβ25-35-Induced Autophagy and Cognitive Impairment via the mTOR/TFEB Signaling Pathway. Drug Des Devel Ther. 2020 May 4;14:1705-1716. WB ; Human.

PubMed:3288096

[IF=3.414] Ma HX et al. Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway. J Ethnopharmacol. 2020 Jan 22;252:112583. ICF&WB ; Rattus norvegicus.

PubMed:31978519

[IF=4.2] Yang, Huan, et al. "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." Stem Cell Research & Therapy 8.1 (2017): 267. WB ; Human.

PubMed:29157289

[IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801 WB ; Human.

PubMed:25596736

[IF=2.33] Tang, Qishan, et al. "Resveratrol-induced apoptosis is enhanced by inhibition of autophagy in esophageal squamous cell carcinoma." Cancer letters 336.2 (2013): 325-337. WB ; Human.

PubMed:23541682