Rabbit Anti-SRF antibody | Sunlong Medical

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c fos serum response element binding factor; Serum Response Factor SRF; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM-1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum r

Cat:

SL1401R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human SRF:101-250/508

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: NIH/3T3 Cell Lysate at 30 ugPrimary: Anti-SRF (SL1401R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 56 kDObserved band size: 54 kDSample: Muscle(Mouse) Lysate at 30 ugPrimary: Anti- SRF (SL1401R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 56 kDObserved band size: 56 kDSample: Large intestine (Mouse) Lysate at 30 ugPrimary: Anti- SRF (SL1401R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 56 kDObserved band size: 54 kDSample: A431(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ug293T(Human) Cell Lysate at 30 ugPrimary: Anti- SRF (SL1401R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SRF) Polyclonal Antibody, Unconjugated (SL1401R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq, Jul 2008].

Function:
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.

Subunit:
Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1. Forms a nuclear ternary complex with MKL1 and SCAI.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated by PRKDC.

Similarity:
Contains 1 MADS-box domain.

SWISS:
P11831

Gene ID:
6722

Database links:

Entrez Gene: 6722 Human

Entrez Gene: 20807 Mouse

Entrez Gene: 501099 Rat

Omim: 600589 Human

SwissProt: P11831 Human

SwissProt: Q9JM73 Mouse

Unigene: 520140 Human

Unigene: 45044 Mouse

转录调节因子（Transcriptin Regulators）
血清应答因子(SRF)是一种转录因子蛋白，可以结合启动子DNA序列中的血清应答元件(SRE)，调节一系列基因的表达，包括即刻早期基因的表达，如c-fos、fosB、junB、egr-1、egr-2，以及神经元基因nurr1、肌肉基因如肌动蛋白、肌球蛋白基因等. Picture

Sample: NIH/3T3 Cell Lysate at 30 ug
Primary: Anti-SRF (SL1401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 56 kD
Observed band size: 54 kD

Sample: Muscle(Mouse) Lysate at 30 ug
Primary: Anti- SRF (SL1401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample: Large intestine (Mouse) Lysate at 30 ug
Primary: Anti- SRF (SL1401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 56 kD
Observed band size: 54 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- SRF (SL1401R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SRF) Polyclonal Antibody, Unconjugated (SL1401R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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