Rabbit Anti-Cathepsin L antibody

Home > Product > Antibody > Rabbit Anti-Cathepsin L antibody

Cathepsin L heavy chain; Cathepsin L light chain; CathepsinL; CATL; CTSL; CTSL protein; DKFZp686A18159; FLJ31037; Major excreted protein; MEP; cath-L; CL; CATL1_HUMAN; CathepsinL1; Cathepsin L1,Cathepsin-L1; CTSL 1; CTSL-1,CTS L1,CTS-L1.

Cat:

SL1508R

Species Reactivity：

Human,Rat,(predicted: Mouse,)

Immunogen：

KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein:71-170/334

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human HUVEC cell Lysates Lane 2: Human K562 cell Lysates Lane 3: Human MCF-7 cell Lysates Primary: Anti-Cathepsin L (SL1508R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 19/30/37kDaObserved band size: 40kDaSample: A549 Cell (Human) Lysate at 30 ugK562 Cell (Human) Lysate at 30 ugPrimary: Anti- Cathepsin L (SL1508R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 19/30/37 kDObserved band size: 40 kDProtein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug; Primary: Anti-Cathepsin L (SL1508R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 37kDObserved band size : 19kD, 37kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (SL1508R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(SL1508R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated(SL1508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control:A549. Primary Antibody (green line): Rabbit Anti-Cathepsin L antibody (SL1508R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

Function:
Important for the overall degradation of proteins in lysosomes.

Subunit:
Dimer of a heavy and a light chain linked by disulfide bonds.

Subcellular Location:
Lysosome.

Similarity:
Belongs to the peptidase C1 family.

SWISS:
P07711

Gene ID:
1514

Database links:

Entrez Gene: 1514 Human

Entrez Gene: 13039 Mouse

Entrez Gene: 25697 Rat

Omim: 116176 Human

SwissProt: P07711 Human

SwissProt: P06797 Mouse

SwissProt: P07154 Rat

Unigene: 726015 Human

Unigene: 930 Mouse

Unigene: 1294 Rat

合成与降解（Synthesis and Degradation）
组织蛋白酶L(cathepsin L CL)属于木瓜蛋白酶家族中的半胱氨酸蛋白水解酶，以酶原的形式贮存于溶酶体中. 参与许多特殊的生理过程，如激素原的激活、抗原呈递、组织器官的发育等。
在病理状态下，因各种原因所致的细胞损伤(如病原微生物、炎症因子、氧化应激等)可降解细胞成分或细胞间质基质成分，包括层粘素、IV型胶原纤维及纤维连接素等，与人类许多疾病如肿瘤的浸润与转移、关节炎、骨质疏松、阿尔茨海姆病、多发性硬化症及其他慢性炎症性疾病有关. Picture

Sample:
A549 Cell (Human) Lysate at 30 ug
K562 Cell (Human) Lysate at 30 ug
Primary: Anti- Cathepsin L (SL1508R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/30/37 kD
Observed band size: 40 kD

Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug;
Primary: Anti-Cathepsin L (SL1508R) at 1:200;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 37kD
Observed band size : 19kD, 37kD

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (SL1508R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(SL1508R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated(SL1508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control:A549.
Primary Antibody (green line): Rabbit Anti-Cathepsin L antibody (SL1508R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall