Rabbit Anti-ERCC1 antibody | Sunlong Medical

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Excision Repair Cross Complementing 1; DNA excision repair protein ERCC-1; COFS4; UV20; DNA excision repair protein ERCC1; ERCC 1; Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1; Excision repair protein; UV 20; UV20;D

Cat:

SL1726R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human ERCC1:151-250/297

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti-ERCC1 (SL1726R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 35 kDSample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-ERCC1 (SL1726R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 35 kDParaformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERCC1) Polyclonal Antibody, Unconjugated (SL1726R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERCC1) Polyclonal Antibody, Unconjugated (SL1726R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ERCC1 Polyclonal Antibody, Unconjugated(SL1726R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Subunit:
Heterodimer composed of ERCC1 and XPF/ERRC4.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the ERCC1/RAD10/SWI10 family.

SWISS:
P07992

Gene ID:
2067

Database links:

Entrez Gene: 2067 Human

Entrez Gene: 13870 Mouse

Entrez Gene: 292673 Rat

Omim: 126380 Human

SwissProt: P07992 Human

SwissProt: P07903 Mouse

Unigene: 435981 Human

Unigene: 280913 Mouse

Unigene: 764 Rat

ERCC1蛋白是动物内重要的DNA修复基因家族成员之一。在人体内它的低表达往往伴随着基因不稳定性增加,从而产生肿瘤恶性变的发生。ERCC1是损伤DNA核苷酸切除修复中高度保守的切除性核酶。在哺乳动物的核苷酸切除修复中，XPG负责切割DNA损伤区域的3’端，而ERCC1-XPF复合物在5’端打开切口。目前初步的研究表明，癌瘤中的ERCC1蛋白表达程度越低，顺氯氨铂化疗的效果就越好。ERCC1蛋白阳性的患者，非化疗治疗的预后要好于接受化疗。 Picture

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-ERCC1 (SL1726R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35 kD

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERCC1) Polyclonal Antibody, Unconjugated (SL1726R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERCC1) Polyclonal Antibody, Unconjugated (SL1726R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ERCC1 Polyclonal Antibody, Unconjugated(SL1726R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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