Rabbit Anti-Angiotensin II type 1A receptor antibody

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AGTR1; Agtr1a; AT1; AT1A; AT1AR; Type 1 angiotensin II receptor; AGTR1_HUMAN; AGTR1B; AT2R1; AT2R1B.

Cat:

SL2132R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human Angiotensin II type 1A receptor:101-200/359<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugLung (Mouse) Lysate at 40 ugAdrenal gland (Rat) Lysate at 40 ugPrimary: Anti-Angiotensin II type 1A receptor (SL2132R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 56 kDSample: A549(Human) Cell Lysate at 430 ugPrimary: Anti-Angiotensin II type 1A receptor (SL2132R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 41/51 kDSample: Lane 1: Liver (Mouse) Lysate at 40 ugLane 2: Heart (Mouse) Lysate at 40 ugLane 3: Placenta (Mouse) Lysate at 40 ugLane 4: Kidney (Mouse) Lysate at 40 ugLane 5: Embryo (Mouse) Lysate at 40 ugLane 6: Cerebrum (Mouse) Lysate at 40 ugLane 7: NIH/3T3 (Mouse) cell Lysate at 30 ugLane 8: Liver (Rat) Lysate at 40 ugLane 9: Heart (Rat) Lysate at 40 ugLane 10: Kidney (Rat) Lysate at 40 ugLane 11: Cerebrum (Rat) Lysate at 40 ugLane 12: Embryo (Rat) Lysate at 40 ugPrimary: Anti-Angiotensin II type 1A receptor (SL2132R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 43 kDObserved band size: 46 kDSample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti- Angiotensin II type 1A receptor (SL2132R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 42 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (SL2132R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-Angiotensin II type 1A receptor antibody (SL2132R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-Angiotensin II type 1A receptor antibody (SL2132R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Angiotensin II type 1 receptor (AT1) is the primary effector of Angiotensin II, a key regulator of blood pressure and fluid homeostasis. It is involved in pathogenesis of several cardiovascular diseases such as hypertension, cardiac hypertrophy and congestive heart failure. Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). AT1 has three isoforms in rodents: AT1A (359 aa), AT1B (359 aa), and AT1C (177 aa). Rat AT1's are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular, while the SLCterminus is predicted to be cytoplasmic. AT1's are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle.

Function:
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit:
Interacts with MAS1 (Probable). Interacts with ARRB1.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Liver, lung, adrenal and adrenocortical adenomas.

Post-translational modifications:
SLCterminal Ser or Thr residues may be phosphorylated.

DISEASE:
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P30556

Gene ID:
185

Database links:

Entrez Gene: 185 Human

Entrez Gene: 2436 Rat

Entrez Gene: 81638 Rat

Omim: 106165 Human

SwissProt: P30556 Human

SwissProt: P34976 Rabbit

SwissProt: P25095 Rat

SwissProt: P29089 Rat

Unigene: 477887 Human

Unigene: 728754 Human

Picture

Sample:
A549(Human) Cell Lysate at 430 ug
Primary: Anti-Angiotensin II type 1A receptor (SL2132R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41/51 kD

Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Placenta (Mouse) Lysate at 40 ug
Lane 4: Kidney (Mouse) Lysate at 40 ug
Lane 5: Embryo (Mouse) Lysate at 40 ug
Lane 6: Cerebrum (Mouse) Lysate at 40 ug
Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 ug
Lane 8: Liver (Rat) Lysate at 40 ug
Lane 9: Heart (Rat) Lysate at 40 ug
Lane 10: Kidney (Rat) Lysate at 40 ug
Lane 11: Cerebrum (Rat) Lysate at 40 ug
Lane 12: Embryo (Rat) Lysate at 40 ug
Primary: Anti-Angiotensin II type 1A receptor (SL2132R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 46 kD

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- Angiotensin II type 1A receptor (SL2132R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 42 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (SL2132R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-Angiotensin II type 1A receptor antibody (SL2132R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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