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Home > Product > Antibody > Rabbit Anti-TEM1 antibody
Tumor endothelial marker 1 precursor; TEM 1; TEM-1; tumor endothelial marker 1; CD248; Endosialin; Tumor endothelial marker 1; CD164L1; MGC119478; MGC119479. CD164 sialomucin-like 1; CD248_HUMAN.
Cat:
SL2101R
Species Reactivity：
Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human TEM 1:251-350/757<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEM1) Polyclonal Antibody, Unconjugated (SL2101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


The transmembrane glycoprotein Tumor endothelial marker 1 (TEM1) is highly expressed in tumor endothelial cells however it is barely detectable on normal endothelial cells. It is believed to play a role in tumor angiogenesis. It is expressed in stromal fibroblasts, metastatic hepatic lesions and during angiogenesis of corpus luteum formation and wound healing. TEM1 is being investigated as a potential target for cancer treatment.

Function:
May play a role in tumor angiogenesis.

Subcellular Location:
Membrane; Single-pass type I membrane protein (Probable).

Tissue Specificity:
Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.

Post-translational modifications:
O-glycosylated with sialylated oligosaccharides.
May be N-glycosylated.

Similarity:
Contains 1 SLCtype lectin domain.
Contains 1 EGF-like domain.Contains 1 Sushi (CCP/SCR) domain.

SWISS:
Q9HCU0

Gene ID:
57124

Database links:

Entrez Gene: 57124 Human

Entrez Gene: 70445 Mouse

Entrez Gene: 293669 Rat

SwissProt: Q9HCU0 Human

SwissProt: Q91V98 Mouse



内皮唾液酸蛋白（CD248）是近年发现的人类肿瘤标记物之一,在人类多种恶性肿瘤组织中异常高表达,内皮唾液酸蛋白对肿瘤细胞生长、血管生成、浸润及转移有重要作用。 Picture

Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEM1) Polyclonal Antibody, Unconjugated (SL2101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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