This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct SLCterminal sequences have been described. [provided by RefSeq, May 2010]
Function:
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
Subunit:
Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with INADL, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR.
Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endosome. Note=Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
Tissue Specificity:
soform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
Q13639
Gene ID:
372
Database links:
Entrez Gene: 372 Human
Entrez Gene: 15562 Mouse
Entrez Gene: 25324 Rat
GenBank: NP_000861.1 Human
Omim: 602164 Human
SwissProt: O70528 Guinea pig
SwissProt: Q13639 Human
SwissProt: P97288 Mouse
SwissProt: Q5806 Pig
SwissProt: Q62758 Rat
Unigene: 483773 Human
Unigene: 2088 Mouse
Unigene: 10094 Rat
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Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-5HT4 Receptor (SL 2127R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Lane 1: Stomach (Mouse) Tissue Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Tissue Lysate at 40 ug
Lane 3: Heart (Mouse) Tissue Lysate at 40 ug
Lane 4: Adrenal gland (Mouse) Tissue Lysate at 40 ug
Lane 5: Adrenal gland (Rat) Tissue Lysate at 40 ug
Primary: Anti-5HT4 Receptor (SL2127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD
Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-5HT4 Receptor (SL2127R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (SL2127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (SL2127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (SL2127R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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