Rabbit Anti-CLEC7A antibody

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CLC7A_HUMAN; C-type lectin domain family 7 member A; BGR; Beta GR; CLECSF12; DECTIN1; Beta-glucan receptor; beta Glucan Receptor; C-type lectin superfamily member 12; C type calcium dependent carbohydrate recognition domain lectin superfamily member 12; D

Cat:

SL2455R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human CLEC7A:41-140/247<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Lung tissue lysates Primary: Anti-beta Glucan Receptor (SL2455R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDaObserved band size: 28,47 kDaSample: Spleen (Mouse) Lysate at 40 ug Thymus (Mouse) Lysate at 40 ugPrimary: Anti- beta Glucan Receptor (SL2455R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 28 kDSample: Lung(Mouse) lysate at 30ug;Primary: Anti-beta Glucan Receptor (SL2455R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1:5000 dilution; Predicted band size: 28 kDObserved band size: 28 kDBlank control:RAW264.7. Primary Antibody (green line): Rabbit Anti-Iba1 antibody (SL2455R) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-Rabbit IgG-AF488Dilution: 0.5ug/Test. Isotype control（orange line）：Normal Rabbit IgGProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (SL2455R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the SLCtype lectin/SLCtype lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular SLCtype lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq].

Function:
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity).

Subunit:
Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.

Tissue Specificity:
Detected in dendritic cells, in paracortical and medullary regions of lymph nodes, and in spleen red pulp and white pulp.

Post-translational modifications:
on tyrosine residues in response to glucan binding.

Similarity:
Contains 1 SLCtype lectin domain.

SWISS:
Q9BXN2

Gene ID:
64581

Database links:

Entrez Gene: 64581 Human

Entrez Gene: 56644 Mouse

Entrez Gene: 502902 Rat

Omim: 606264 Human

SwissProt: Q9BXN2 Human

SwissProt: Q6QLQ4 Mouse

Unigene: 143929 Human

Unigene: 239516 Mouse

β-葡聚糖受体是一种非调理素依赖性受体,可在免疫反应早期,在清除衰老细胞和防御感染方面发挥重要作用,进而实现其抗肿瘤、抗感染等生物学效应。 Picture

Sample:
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- beta Glucan Receptor (SL2455R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD

Sample: Lung(Mouse) lysate at 30ug;
Primary: Anti-beta Glucan Receptor (SL2455R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1:5000 dilution;
Predicted band size: 28 kD
Observed band size: 28 kD

Blank control:RAW264.7.
Primary Antibody (green line): Rabbit Anti-Iba1 antibody (SL2455R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-Rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (SL2455R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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