Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO NRSF (Human) Cell Lysate at 30 ug
Primary: Anti- NRSF (SL2590R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-NRSF (SL2590R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
NRSF was immunoprecipitated from human hela cells lysate with SL2590R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: SL2590R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(SL2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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