Rabbit Anti-CK18 antibody | Sunlong Medical

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K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18 (CK-18); Keratin-18 (K18); PIG46; Keratin 18;

Cat:

SL2043R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CK18:53-150/430

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Large intestine (Rat) Lysate at 40 ug Primary: Anti- Cytokeratin 18 (SL2043R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Sample: LOVO(Human) Cell Lysate at 30 ugPrimary: Anti- Cytokeratin 18 (SL2043R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 63 kDSample: Large intestine (Mouse) Lysate at 40 ugPrimary: Anti-Cytokeratin 18 (SL2043R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 48 kDParaformaldehyde-fixed, paraffin embedded (Human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 18) Polyclonal Antibody, Unconjugated (SL2043R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 18) polyclonal Antibody, Unconjugated (SL2043R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Cell: PSLC12Concentration:1:100Host/Isotype:Rabbit/IgGFlow cytometric analysis of Rabbit IgG isotype control (Cat#: SL2043R) on PSLC12(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Subunit:
Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with the thrombin-antithrombin complex. Interacts with DNAJB6, TCHP and TRADD (By similarity).

Subcellular Location:
Cytoplasm, perinuclear region. Nucleus, nucleolus.

Tissue Specificity:
Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues.

Post-translational modifications:
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

DISEASE:
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

Similarity:
Belongs to the intermediate filament family.

SWISS:
P05783

Gene ID:
3875

Database links:

Entrez Gene: 50696 Cow

Entrez Gene: 477601 Dog

Entrez Gene: 3875 Human

Entrez Gene: 16668 Mouse

Entrez Gene: 294853 Rat

SwissProt: P05783 Human

SwissProt: P05784 Mouse

SwissProt: Q5BJY9 Rat

结构蛋白（Structural Proteins）常用于肿瘤细胞的分化、增殖及转移方面的研究。 Picture

Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti- Cytokeratin 18 (SL2043R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 63 kD

Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-Cytokeratin 18 (SL2043R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Paraformaldehyde-fixed, paraffin embedded (Human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 18) Polyclonal Antibody, Unconjugated (SL2043R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 18) polyclonal Antibody, Unconjugated (SL2043R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Cell: PSLC12
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: SL2043R) on PSLC12(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

Product Feedback Wall

Specific References (11) | SL2043R has been referenced in 11 publications.

[IF=6.543] Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022 WB,IF ; Rat,Human.

PubMed:35308169

[IF=4.59] Fagundes et al. Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats. (2016) Oxid.Med.Cell.Longe. 2016:7323627 IHSLCP ; Rat.

PubMed:27579155

[IF=6.543] Zhong Heju. et al. Methionine Protects Mammary Cells against Oxidative Stress through Producing S-Adenosylmethionine to Maintain mTORC1 Signaling Activity. Oxid Med Cell Longev. 2021;2021:5550196 FC ; Pig.

PubMed:3437298

[IF=1.953] Liu et al. Therapeutic effects of suppressors of cytokine signaling in diabetic nephropathy. (2014) J.Histochem.Cytoche. 62:119-28 WB, IHSLCP ; Mouse.

PubMed:24217922

[IF=2.766] Li et al. Hyperglycaemia exacerbates choroidal neovascularisation in mice via the oxidative stress-induced activation of STAT3 signalling in RPE cells. (2012) PLoS.One. 7:e47600 IF ; Mouse.

PubMed:23094067

[IF=5.88] Fei Sun. et al. Directly construct microvascularization of tissue engineering trachea in orthotopic transplantation. Mat Sci Eng SLCMater. 2021 May;:112201 IHC ; Rabbit.

PubMed:10.1016/j.msec.2021.112201

[IF=4.486] Yu Zhong. et al. LIGHT aggravates sepsis‐associated acute kidney injury via TLR4‐MyD88‐NF‐κB pathway. J Cell Mol Med. 2020 Oct;24(20):11936-11948 IF ; Human.

PubMed:32881263

[IF=3.73] Zeng, Wei-qun, et al. "A New Method to Isolate and Culture Rat Kupffer Cells." PLOS ONE 8.8 (2013): e70832. Rat.

PubMed:23967115

[IF=2.332] Gao R et al. Protective effect of chlorogenic acid on lipopolysaccharide-induced inflammatory response in dairy mammary epithelial cells.Microb Pathog. 2018 Jul 24;124:178-182. WB ; bovine.

PubMed:3005724

PubMed:27579155

[IF=3.73] Liu, Pengfei, et al. "Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy." PLoS One 9.5 (2014): e97123. Rat.

PubMed:24824427